RTase Efficiency ?

Aydemir Akin akin at sage.cc.purdue.edu
Sat Jul 29 05:50:03 EST 1995


Does anyone have some good references on the efficiency of reverse
transcription reactions (that is conversion rate of ssRNA/dsRNA to cDNA)? 

In my (very) limited experience, it seems to be around 7-10%.  Do you
think this is TOO low.  Any inputs (experience) would be appreciated.  

Thanks,  :­)

Aydemir Akin, D.V.M.
1175 Animal Disease Diagnostic Lab
Purdue University
West Lafayette, IN 47907-1175

akin at sage.cc.purdue.edu

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