seemingly two bands of one band in SDS-PAGE?

Hanbok Kim hbkim at
Sun Jul 30 06:53:52 EST 1995

Separation of protein bands of higher M.W ( 60 kDa) is 
ok, however that of lower one (25 kDa) is strange sometimes
in that it has two forms of bands rather than one in 
SDS-PAGE. One band is slightly lower than the other. To
my knowledge, proteolysis of the band (25 kDa) does not
occur. Is this phenomenon artefact? How can I prevent it?

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