HELP -DNA extraction from phage

Daniel Kim dkim at nmsu.edu
Thu Jul 27 13:44:34 EST 1995


Hello:

WRT extracting phage DNA.  Does the Bio101 kit you are using have a 
Geneclean-like step?  That is, a glassmilk binding step?  If so, you may 
be getting shearing of the bound DNA, if it is bound to more than one 
glass particle at a time.  Shearing may also become a problem when doing 
extractions in which vigorous agitation (e.g. vortexing) is used.

Daniel Kim




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