(none)

[Francisco G da Nobrega ib - bio 7588]

Dear netters:
We are having problems in cloning a PCR amplified yeast gene into an 
expression vector. In one case site is Bgl II in one primer and Xba I in 
the other both with only 2 nucleotides from the edge of fragment. In the 
other case we have BamH I sites with 3 extra nucleotides from the edge. 
Any suggestions?  Could it be because sites are too near edges?
		F.G.Nobrega       fgdnobre at usp.br