PCR of lambda gt10

chihara chihara at alm.usfca.edu
Mon Jul 31 18:23:56 EST 1995


O, here's the cycle: (denaturation)
10 min at 95 deg.
35 cycles: 30 sec 95 deg.
30 secs. 52 deg.(annealing)
1 min 72daeg. 
1 cycle 10 min 72 deg. (extension)
Good luck. 





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