GETTING RID OF EtBr
u7k0201 at sunmail.lrz-muenchen.de
Mon Jul 31 13:50:36 EST 1995
Wolfgang Schechinger <u7k0201 at sunmail.lrz-muenchen.de> wrote:
>gbga13 at udcf.gla.ac.uk (B.L.Cohen) wrote:
>>In article <3v5rvf$jro at sparcserver.lrz-muenchen.de>, Wolfgang Schechinger
>><u7k0201 at sunmail.lrz-muenchen.de> wrote:
>>> Hmm. This point of interest comes up again and again...
>>> If you have the facility of bringig it to a suitable incinerator (infectious waste e.g.) do
>>> this. Or use at least 1M sodium hydroxide. This will destroy it. (You can measure this by
>>Can you please provide a reference to the use of alkali to detoxify EtBr?
>>It is a suggestion that I have not seen before and might be useful.
>>But can any chemists indicate what the products might be?
>>Remember that the product of hypochlorite treatment of EtBr is mutagenic in
>>the Ames test _without_ microsomal activation and may therefore be a more
>>potent mutagen in vivo (mammals) that the unaltered compound (even though
>>there may be very little of it).
>Sorry, but I cannot give you a reference. If we have small amounts of EtBr in a solution, we
>mix it with NaOH and put it to our toxic waste. Gelc etc. we do put into a bag that goes to
Well, I must hereby clearly state that this is a 'protocol' that has not been prooved. So I do
not recommend to use it without further proving. My reply to the question should be a
suggestion for solving the problem.
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Institute for Diabetes Reserch
Koelner Platz 1
Phone +49 (89) 30 79 31 24
Fax +49 (89) 30 81 733
email u7k0201 at sunmail.lrz-muenchen.de
(Standard disclaimer applies)
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