Help in SDS-PAGE

Constantin Polychronakos mc97 at musica.McGill.CA
Sat Jul 29 12:45:11 EST 1995

>   Giorgio Spagnol <spagnol at> writes:
>        ... instead of migrating as bands, my {SDS-PAGE} sample
>  form two vertical streaks wich seem to migrate from the sides of the
>  wells down in the gel.
>  Giorgio

	Sounds like the bottom of your wells is clogged. Are you sure there is 
absolutely no particulate matter in your samples?

Constantin Polychronakos
Department of Pediatrics
McGill University, Montreal

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