PCR artifacts

rcha244 at bay.cc.kcl.ac.uk rcha244 at bay.cc.kcl.ac.uk
Mon Jul 31 12:44:33 EST 1995


I am having some unusual pcr artifacts with a batch of taq from a new 
supplier. I consistently get a ladder effect up to the major band which 
is of expected size. I have tried various titrations of DNA, Mg, primers 
and Taq which dont eliminate the problem entirely. The pcr is a 
reampification of them same fragment. I have also seen it with RTPCR 
products. Any suggestions?

Paul Crossey 
Dept of Medicine
KCSMD
London





More information about the Methods mailing list