PCR of lambda gt10
jfhess at ucdavis.edu
Mon Jul 31 12:42:40 EST 1995
Kevin Lease asked about PCR of lambda gt10.
My experience is with gt 11 and now, charon 21a. In each case, I haven't
had problems (and in fact have had good success) starting things off with
a 1 minute 94 degree step. Then PCR as usual. These conditions work for
phages and ecolis. I haven't tried them with yeast but may very soon.
John Hess, PhD Phone me 916 752 8420
Dept of Human Anatomy
University of Calif Email me jfhess at ucdavis.edu
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