PCR of lambda gt10

[jfh]

   Kevin Lease asked about PCR of lambda gt10.


My experience is with gt 11 and now, charon 21a.  In each case, I haven't
had problems (and in fact have had good success) starting things off with
a 1 minute 94 degree step.  Then PCR as usual.  These conditions work for
phages and ecolis.  I haven't tried them with yeast but may very soon.


John Hess, PhD                    Phone me 916 752 8420
Dept of Human Anatomy             
University of Calif               Email me jfhess at ucdavis.edu
Davis, CA