gelatin block for Westerns

[John R. Thompson]

Helen McBride <hmcbride at hlthsci.med.utah.edu> wrote:

>To get gelatin to go into solution, we would always heat a 1% solution
>with stirring and when cooled added .01% Sodium Azide as a preservative
>so we could store the solution at room temperature. We never had any
>problems with it coming out of solution this way. The best block for
>Immobilon that I have found is a combination solution of  1% gelatin with
>2% dry milk.  Good Luck.

>hmcbride at hlthsci. med.utah.edu

I use fish gelatin from SIGMA.  Remains liquid at room temp. Comes as a
concentrated liquid solution.

Yours, 
John R. Thompson
jrt at intac.com
Merck Research Labs