gelatin block for Westerns

John R. Thompson jrt at
Mon Jul 31 21:05:23 EST 1995

Helen McBride <hmcbride at> wrote:

>To get gelatin to go into solution, we would always heat a 1% solution
>with stirring and when cooled added .01% Sodium Azide as a preservative
>so we could store the solution at room temperature. We never had any
>problems with it coming out of solution this way. The best block for
>Immobilon that I have found is a combination solution of  1% gelatin with
>2% dry milk.  Good Luck.

>hmcbride at hlthsci.

I use fish gelatin from SIGMA.  Remains liquid at room temp. Comes as a
concentrated liquid solution.

John R. Thompson
jrt at
Merck Research Labs

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