How to detect GFP via FACS?

[Said Hashemolhossein]

Hi,

  I try to detect GFP after transfection in NIH3T3 or 293 without
success.  Transfection is for sure not the problem ( I am able to
transfect with luciferase + detect in luminometer ). 
  I want to detect the
GFP containing cells via FACS (Becton Dickinson , FL1H / FL2A) , but
cannot see anything.
  Even under the microscope it is not possible to
detect positive cells. 


Does anyone can help???....


thanks a lot ....   


said


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Dr. Said Hashemolhosseini	phone (761)2061531
Tumor Biology Center		fax   (761)2061505
Dep Exp Cancer Research		email said at sun1.tumorbio.uni-freiburg.de
Breisacherstr. 117
D-79106 Freiburg, Germany
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