Methods July 1995 Archives by author

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Starting: Sat Jul 1 08:02:28 EST 1995
Ending: Mon Jul 31 23:25:01 EST 1995
Messages: 1250

Help! - EMS mutagenesis "Marianne Leverone ", BIO
how to make skim milk soluble? "Marianne Leverone ", BIO
Direct PCR from E. coli colonies B VISSER * x2818 Plantkunde *
PCR cloning
Req: Info. on 2D-SDS-PAGE
Coomassie related question
Ultra-low freezer recommendation
in vitro tranlation of retroviral gene
test, please ignore. Grad Student Lab 1
help needed about western blots Grad Student Lab 1
Sequencing gel solution premixes R. P. Grant +44 1 865 221018
Sma I religation problem R. P. Grant +44 1 865 221018
Sma I / PEG R. P. Grant +44 1 865 221018
Sma I religation - NO PROBLEM! R. P. Grant +44 1 865 221018
Sequencing gel solution premixes R. P. Grant +44 1 865 221018
Plasmid loss after restreaking R. P. Grant +44 1 865 221018
Temp of Dry ice? (ie sublimation point) R. P. Grant +44 1 865 221018
UK phone no. for Bio/Gene E.L. Newman Surgery ext ninewells 3174
wanted: mouse cDNA library 3EUK at QUCDN.QueensU.CA
hekp with sigmacoat and sequencing gels 4700gbera at umbsky.cc.umb.edu
pcr from E. coli colonies 4700gbera at umbsky.cc.umb.edu
(none) Francisco G da Nobrega ib - bio 7588
(none) HOFSTRA ANGELA 905-822-3520
Glucose oxidase at
northerns at
Hydrophobic Peptide: Fragment Detection A999999361
Request help with p53 AR.Dallol at ulst.ac.uk
Automated DNA Extractors Gregory M. Acland
Automated DNA extractors Gregory M. Acland
microfuge swinging bucket rotor?? David A. Adler
New net.bio.net IP address still not propagated correctly! BIOSCI Administrator
Yet Another net.bio.net Progress Report ... BIOSCI Administrator
PROGRESS REPORT - ALL CLEAR!!!! BIOSCI Administrator
Molecular Dynamics and Knight-Ridder Information sponsor BIOSCI!! BIOSCI Administrator
UNSUBSCRIBING, BIOSCI ARCHIVES, ADDRESS DATABASE & BIOSCI FAQ BIOSCI Administrator
DDRT-PCR Northerns Christoph Ahlers
Home-Made TA cloning Aydemir Akin
RTase Efficiency ? Aydemir Akin
RDA Tina Albertson
RDA Tina Albertson
RDA Tina Albertson
Home-Made TA cloning Shane Albright
Lysing yeast spheroplasts Nancy Alexander
RNA gels - staining and denaturing Nancy Alexander
Differential Display by RT/PCR Nancy Alexander
PCR sequencing Danny Altshuler
PCR on fresh plant tissue? Paul St. Amand
Ligation in 5 min! Eric C. Anderson
protein concetration Eric C. Anderson
DNA recovery from gels Eric C. Anderson
UV Sterilization Eric C. Anderson
how about bionet.molbio.pcr? Eric C. Anderson
direct sequencing Eric C. Anderson
his-tagged protein purification with metal chelate columns Eric C. Anderson
Plasmid sequencing: Methods to improve resolution Roger Anderson
Sma I religation problem Roger Anderson
Schleicher & Schuell,Inc phone number?????? Roger Anderson
Calculating Tm - Help Needed Roger Anderson
Calculating Tm - Help Needed Roger Anderson
Catalogs: VWR,SarWel,Grainger,McMaster,LabLine.. Roger Anderson
Recommendations for software to support small sequencing project Dr Alexander J Annala
Where are codon pref. tables? Tracy Aquilla
Southern Hybridization Buffers? Tracy Aquilla
Does RNA - PCR ?? Tracy Aquilla
Any BIO-RAD email, gopher, or WWW address? Tracy Aquilla
Users of BIO-RAD Molecular Imager Systems... Tracy Aquilla
Any BIO-RAD email, gopher, or WWW address? Tracy Aquilla
Designing PCR primers-How to check them? Tracy Aquilla
Northern blotting Tracy Aquilla
Does RNA - PCR ?? Tracy Aquilla
Plasmid prep'ing machine Graham Atherton
Antisense oligo experiments - help Graham Atherton
silicon carbide whisker Charles F Austerberry
GETTING RID OF EtBr B.L.Cohen
**** CsSO4 Step gradients for RNA...**** B.Schonhoft
pET3a B.Schonhoft
RT-PCR and gels GORDON BETTS
Call for LacZ fragment for terminal bash Dina Raveh BGU
Preparing competent JM109 BOIS, DANUTA H
Sma I religation problem Amadou Ba
Mammalian Electroporation?!? Dr Jonathan Backer
Sealing GFP coverslips Jon Backer
hekp with sigmacoat and sequencing gels Mike Barnes
ICI Seeds address Mike Barnes
Help! - EMS mutagenesis Tim Barnett
DMSO in CaCl2 Competent Cells Tim Barnett
DNA sticking to glass Chris Barry
Intelligenetics Bart.Nelissen
Primer Sets for Linkage Analysis Dr David E Barton
ALF Woes! Dr David E Barton
PCR from blood Dr David E Barton
Thermocyclers Dr David E Barton
dna prep for eukaryotic cells (macrophages)? Dr David E Barton
Expression of GFP in eukaryotic cells. Dave Bates
SOURCE OF LUCIFERASE John Bates
pCDNAI and pCDM8 problems in stable transfection ? Clifford Beall
Tm calculation and software Anderas Becker
3kB PCR Cycling Conditions?// Anderas Becker
how about bionet.molbio.pcr? Anderas Becker
PCR to detect viral point mutations Anderas Becker
[Q] L-amino acid kits Bob Belas
how to make skim milk soluble? Bob Belas
Apa1 digestion Daniel Berman
Experience with pZERO-1 Bernard
Request a front-end to GeneBank for PC's Bernard
GETTING RID OF EtBr Gordon Betts
Photographing gels Gordon Betts
Triethylamine Dr. S. Bhattacharya
Protein elution from Ab columns Shoumo Bhattacharya
Yeasy PCR screening problem Shoumo Bhattacharya
PCR Mutagenesis Shoumo Bhattacharya
Plasmid vector with adenoviral E1a gene needed! Institute for Biochemistry
how about bionet.molbio.pcr? Vasos Panagiotopoulos +1-212-287-8087 Bioengineer-Financier
ECL and cDNA cloning Morris J. Birnbaum
A Source of Company email/WWW addresses Shaun D. Black
Immunoblot sensitivity Martin Blankfard
E. coli transformation Sean Blasdall
Cheap Biotin labeled tRNA's Darren Boehning
protein concetration Darren Boehning
Temp of Dry ice? (ie sublimation point) Darren Boehning
extracellular chymotrypsin digestion Cynthia Bolognese
prokaryotic 2-hybrid? Peta C. Bonham-Smith
3kB PCR Cycling Conditions?// Marc A. Borbely
In vitro mutagenesis with rec A Jonathan Bown
Coating LB plates with tet after pouring? Chris Boyd
Sma I religation problem Chris Boyd
How to remove albumin? Tomas Bratt
How to control the PCR where genomic DNA as a template G. Briscoe
Deoxyinosine or mixed bases , whis is better? G. Briscoe
Address of HT Biotechnology? D. Brittain
Address of SphaeroQ? D. Brittain
Help: Blotting DNA from acrylamide gel Stuart Brown
RNA gels - staining and denaturing Darren D. Browning
peptide synthesis Darren D. Browning
Cat Ig mRNA detection Sebastian Bunka
electroporation of BL21(DE3)pLysS CDMENU at ccs1.cc.monash.edu.au
Lysing yeast spheroplasts Manuel .G CLAROS
how to work out curvature of DNA??? Laurentiu COCEA
Calculating Tm - Help Needed Laurentiu COCEA
Mitomycin C Andrew Cannons
exposing tritium gels Fiona Cassidy
Library woes... Barry Causier
DMSO in CaCl2 Competent Cells Ivica Cerina
Stability of Luc or GFP Protein Daniel Chagnovich
Is there a shareware plasmid drawing program? Dhruba Chakravarti
Plant mRNA isolation protocol William Woei-Ping Chang
source for anti-HA Tom Chappell
PCR pipetors Tom Chappell
Why probe hybridizes to vector? Mic Chaudoir
myosin purification Mic Chaudoir
B/W Photos of Westerns ? Mic Chaudoir
Help needed in screening libraries. Mic Chaudoir
Lambda Hind III Marker Does anyone know of a cheap source for Lambda Hind III? Paying over $100 for 500 ug seems a little steep. Don Chen
Taq polymerase from Fisher Hemant Chickarmane
6-N-Propyl-2-Thiouracil Derrick Chilton
Who sells Gilson pipettors? Jaishree Muppala Chittoor
Electrophoresis newsgroup? Peter Chowla
selective pressure on E. coli stored on LB plate in lab fridge Chris=Michiels%LMM%AGR at agr.kuleuven.ac.be
hekp with sigmacoat and sequencing gels Thomas Chuang
pT7-7 cloning Chunlin
Home-Made TA cloning Duncan Clark
Home-Made TA cloning Duncan Clark
Calculating Tm - Help Needed Duncan Clark
Does RNA - PCR ?? Duncan Clark
ATCC book on freeze drying methods Duncan Clark
ATCC book on freeze drying methods Duncan Clark
Does RNA - PCR ?? Duncan Clark
Accurate and Long PCR Michael Clark
Transcription start site determination. D L Clarke
pET3a Ian Clarke
Help - Spotted Blots Robert Cobuzzi
Luciferase/CAT assay cell extracts? Cocea
Storage of DNA Clay Comstock
Cost estimates of PCR-based markers vs. RFLP Vergel Concibido
RAPD Problems - Control lanes with bands Michael Cooley
Help needed in screening libraries. Steve Coon
Heterologous protein expression in the yeast Pichia Antony Cooper
384 pin tool for biomek 1000 Alex Copeland
source for anti-HA Seth Corey
Artefacts on SDS PA gels Scott Cowan
HepG2 nuclear extract CrouseJ
Primer Dimers in PCR Ron Croy
Primer Dimers in PCR Ron Croy
Primer Dimers in PCR Ron Croy
MAGNETIC POROUS GLASS (MPG0 DNABILL
LOOKING FOR COLABORATIONS FOR NEW MAGNETIC POROUS GLASS TECNOLOGY DNABILL
DNA sticking to glass DNABILL
PCR Problem DNABILL
WANTED: HELP COATING <uM PARTICLES DNABILL
How to control the PCR where genomic DNA DNABILL
Q on Antisense-Oligo Design DNABILL
immobilizing antibodies, peptides and oth DNABILL
Plant mRNA isolation protocol DNABILL
CPG INC NEED TELEHONE OR FAX DNABILL
[REQ] Cleaning electroporation cuvettes? STEPHEN WILLIAM DOUGHTY
amp resistance gene R Daines
Cloning "Long Range" PCR products Mike Dalrymple
Alternative to Zeocin? DavidM7643
DMSO in CaCl2 Competent Cells Greg Davis
biological/biomedical journal rankings: available on the net? Dino DeAngelis
DNA extraction from fungal mycelia Yeshitila Degefu
Direct PCR from E. coli colonies Graham Dellaire
Southern-PCR Graham Dellaire
Electroporation Challenge!!! Graham Dellaire
Mammalian Electroporation?!? Graham Dellaire
Designing PCR primers-How to check them? Graham Dellaire
Plasmid loss after restreaking Graham Dellaire
PCR GC-rich genomic DNA Graham Dellaire
What is retrotransposon? Graham Dellaire
[Q] How to purify small PCR frg. ? Graham Dellaire
DMSO in CaCl2 Competent Cells Graham Dellaire
Anyone have any GOOD FTP/WEB sites for Biology Freeware/Shareware Graham Dellaire
help X-gal R. Rex Denton
Home-Made TA cloning Brian Desany
Apa1 digestion Susan M. Desimone
SOB Medium: Why add Mg late? Michael Deyholos
Purifying Bacto-Agar: Suggestions? Michael Deyholos
Library screening (non-radioactive) Linda DiPersio
Library Screening (non-radioactive) Linda DiPersio
PCR pipetors Linda DiPersio
rabbit-elisa-assays Val Dias
Is lambda EMBL 3A suppressed by supE? John Dixon
losing overhangs over time? John Dixon
Help needed in screening libraries. John Dixon
PCR in ten minutes! John Dixon
Palindrome and ligations John Dixon
<None> John Dixon
pET3a Andrew Doidd
Somatic in-vivo adenovirus gene therapy Andrew Doidd
384 pin tool for biomek 1000 Maureen Dolan
ICI products Guivarc_h Dominic
Help Biochemical Products Guivarc_h Dominic
Help! Qunfeng Dong
anti c-myc antibody needed? Jen Douglas
Commercial source of X-glu? Tim Doyle
How to purify small PCR frg. ? Try 8% acrylamide gel. Dr.
CAT assay question Harold Drabkin
glycerol Harold Drabkin
ABI DNA Software 2.1.0/PowerMac/HP1200C Carla Drebing
Sma I religation problem Tim Dunn
LacZ vector without nls Duperray
Looking for a LacZ reporter vector without nuclear localisation sequence Alain Duperray
Help with searching promoter sequences for transcription factor recognition sequences EILEEN
Sma I religation problem Mad Dan Eccles
Temp of Dry ice? (ie sublimation point) Mad Dan Eccles
Sma I religation problem Mad Dan Eccles
Wanted:Anti-LacI or HSV-VP16 Edward
Req: Info. on 2D-SDS-PAGE Edward
PCR on fresh plant tissue? SG Edwards
Sealing GFP coverslips Heinz Eipel
Optimal conditions for PCR of small inserts Oudam Em
Taq polymerase from Fisher Jane Q. Employee
GST fusion prots truncating at 27 kDa? Lisa Estey
GST fusion prots truncating at 27 kDa? Lisa Estey
GST fusion prots truncating at 27 kDa? Lisa Estey
GST fusion prots truncating at 27 kDa? Lisa Estey
GST fusion prots truncating at 27 kDa? Lisa Estey
384 pin tool for biomek 1000 Glibert Fabrice
infor. on PCR machine Hung Fang
Help with searching promoter sequences for transcription factor recognition sequences Mr SMNN Faruque
Pre-flashing films for ECL?? Darren Fast
In vitro transcription, Ambion's secret? Eric A. First
hekp with sigmacoat and sequencing gels Karl Fischer
mapping the transcriptional start site: HELP!! Karl Fischer
TA cloning problems!!!! Robert Fisher
Source for sCD14 John T. Flynn
myosin purification Mr A.M. Flynn
SV40, large T antigen and transformed cells??? Alistair Forrest
Source of oligos for repeats in plants M. Fortin
Auxotrophic Mutants John Fox
Custom DNA sequencing John E. Fox
How much protein needed for sequencing John E. Fox
HPLC of L-amino acids John E. Fox
ABI DNA Software 2.1.0/PowerMac/HP1200C John E. Fox
Sma I religation problem Antonio Rodriguez Franco
Sma I religation problem Antonio Rodriguez Franco
Electroporation Challenge!!! Antonio Rodriguez Franco
5-FOA solubility Christian Fritze
Ultra-low freezer recommendation Jim Funderburgh
Accurate and Long PCR Anthony V. Furano
RNA gels - staining and denaturing Anthony V. Furano
Help: Blotting DNA from acrylamide gel Anthony V. Furano
PCR to detect viral point mutations G
how to work out curvature of DNA??? G Gallagher
how to work out curvature of DNA--part two??? G Gallagher
research asst, gene therapy rheumatoid arthritis G Gallagher
IL-10/chromosome .1 G Gallagher
Run-on using DIG-labeled probes Olivier Gandrillon
Where are codon pref. tables? Olivier Gandrillon
Where are codon pref. tables? Olivier Gandrillon
PCR on fresh plant tissue? Olivier Gandrillon
Does RNA - PCR ?? Olivier Gandrillon
Help with searching promoter sequences for transcription factor recognition sequences Olivier Gandrillon
WANTED: biologists' opinions on Internet information sources Olivier Gandrillon
PCR in ten minutes! Frederick Garbrecht
Calculating Tm - Help Needed Mark D. Garfinkel
Photographing gels Mark D. Garfinkel
Calculating Tm for mismatch on Northern Mark D. Garfinkel
Need ideas for Lac+/- screening in Neisseria Mark D. Garfinkel
How to import MD PhosphorImager images? John M. Garrison
Best way to get DNA from gel bands? Allen Gathman
Sequencing gel solution premixes Mike Gaunt
Company consultant stalks this board Mike Gaunt
Urgent Help! French & Italian Electrophoresis Scient - WARNING Mike Gaunt
San Diego Molecular Biology Conf R. Dana - Green Gene
San Diego Molecular Biology Conf R. Dana - Green Gene
RNA/DNA/PROTEIN FROM SAME SAMPLE?? R. Dana - Green Gene
Plant DNA/RNA Isolations R. Dana - Green Gene
Differences in Genomic DNA P R. Dana - Green Gene
help needed at Mol Bio Conf R. Dana - Green Gene
help needed at Mol Bio Conf R. Dana - Green Gene
help needed at Mol Bio Conf R. Dana - Green Gene
how to work out curvature of DNA??? Szatmari George
SOB Medium: Why add Mg late? Szatmari George
[Q] L-amino acid kits Szatmari George
DNA purification from agarose gels Michael Gerdes
Bionet USENET Articles (Search GOPHER) Don Gilbert
SeqPup for MSWin/Mac/XWin (Re: DNA Strider for PCs) Don Gilbert
Ligation in 5 min! Jennifer Glick
PCR GC-rich genomic DNA Steven Goldberg
GC rich riboprobes David Goldstein
Chemiluminescent substrate for alk-phos? David Goldstein
Help stripping non-radioactive northerns Celedonio Gonzalez
What is retrotransposon? Anton Scott Goustin
exposing tritium gels Anton Scott Goustin
dna prep for eukaryotic cells (macrophages)? Anton Scott Goustin
Looking for a computer program Anton Scott Goustin
Looking for a computer program Anton Scott Goustin
Taq polymerase from Fisher Anton Scott Goustin
luciferase assay Anton Scott Goustin
Help with searching promoter sequences for transcription factor recognition sequences Anton Scott Goustin
Calculating Tm for mismatch on Northern Anton Scott Goustin
Recommendations for software to support small sequencing project Anton Scott Goustin
help: cloning oligos Ian Graham
WWW address for John Innes Center Alastair Grant
Avanti Polar Lipids ? Patrick Grealish
E coli expression of eucaryotic protein Patrick Grealish
Pharmacia UK email address? T. S. Grewal
Hydrophobic protein purification Tina Guina
Nucleotide excision repair Chandrasekhar Gujuluva
How much protein needed for sequencing Xin Guo
cleaning up IEF tubes? Chaan Gurer
HELP: Why the PAGE gel cracks sometimes? Chaan Gurer
Electrophoresis newsgroup? Chaan Gurer
How much protein needed for sequencing Chaan Gurer
Disappearance of high molecular weight proteins on 2D gels? Chaan Gurer
HELP-rearranged genomic subclones Michael F. Gurish
B. megaterium rec. DNA guidlines? HARDIES at THORIN.UTHSCSA.EDU
Calculating Tm - Help Needed HARDIES at THORIN.UTHSCSA.EDU
Calculating Tm - Help Needed HARDIES at THORIN.UTHSCSA.EDU
Calculating Tm - Help Needed HARDIES at THORIN.UTHSCSA.EDU
PCR problem (LOH assay) HARDIES at THORIN.UTHSCSA.EDU
PCR GC-rich genomic DNA HARDIES at THORIN.UTHSCSA.EDU
Sequencing AT rich regions Shin-ichiro HIRAGA
Sequencing AT rich regions Shin-ichiro HIRAGA
HELP!! - ExoIII-mung bean digests Shin-ichiro HIRAGA
DMSO in CaCl2 Competent Cells Kenneth HOWE
Human serum albumin sequence? Yoonsoo Hahn
GC rich riboprobes Alan Hair
PCR license for non-profit lab? Andre Hamel
PCR license for diagnostic lab? Andre Hamel
MoAb fragmentation using ficin Yusnainy A. Hamid
MoAb fragmentation using ficin Yusnainy A. Hamid
MoAb fragmentation using ficin Yusnainy A. Hamid
sequencing Sabine Hanelt
How to remove albumin? Kirsten Haraldsen
Does RNA - PCR ?? Richard R. Hardy
ABI DNA Software 2.1.0/PowerMac/HP1200C Richard R. Hardy
GST fusion prots truncating at 27 kDa? Daniel Harkness
Are you from Baylor University?? Oliver Harringer
MicroProbe Corporation? Jean Harris
how to work out curvature of DNA??? Yasha Hartberg
How to detect GFP via FACS? Said Hashemolhossein
Ligations-HELP Burkhard Hassel
ALF Woes! Burkhard Hassel
HELP!! - ExoIII-mung bean digests Burkhard Hassel
PCR Problem Burkhard Hassel
Help needed in screening libraries. Richard Hastings
HELP re: probing phage libraries/hybridisation/+ve controls Richard Hastings
GST fusion prots truncating at 27 kDa? L. Hays
DNA purification from agarose gels L. Hays
Avanti Polar Lipids ? Philip Heacock
Help! pET system not working JG. Head
fungi in the incubator Thorsten Heedt
Rapid delivery of Lectins ?! Thorsten Heedt
Coating LB plates with tet after pouring? Marcus Heller
his-tagged protein purification with metal chelate columns Marcus Heller
Ligation problem Robert Henderson
electroporation of BL21(DE3)pLysS Paul N Hengen
Boomerang DNA Amplification Images Paul N Hengen
Coomassie related question Paul N Hengen
DNA recovery from gels Paul N Hengen
molecular weight of oligos Paul N Hengen
DMSO in CaCl2 Competent Cells Paul N Hengen
random Mutagenesis Paul N Hengen
how about bionet.molbio.pcr? Paul N Hengen
How to clone biotinylated DNA fragment? Paul N Hengen
his-tagged protein purification with metal chelate columns Paul N Hengen
Best way to get DNA from gel bands? Paul N Hengen
Best way to get DNA from gel bands? Paul N Hengen
Consultant company stalks this board Paul N Hengen
DNA recovery from gels Your Name Here
Plant mRNA isolation protocol Your Name Here
Induction by IPTG Bernard Heymann
IPTG and induction, new info Bernard Heymann
SDS-PAGE problem! Andy Hill
tips for removal of GST carrier by thrombin Kiichi Hirota
call for FAX number of dianova GmbH Kiichi Hirota
Supplier of HATU for peptide synthesis E.T. Hitchen
test Yugong Ho
Molbio Suppliers on the Internet - please identify yourselves! Tony Hodge
Calculating Tm - Help Needed Tony Hodge
Photographing gels Tony Hodge
antibodies prep. from inclusion bodies Tony Hodge
Anyone tried Genetrapper-cDNA selection system? Charlie Hoffman
Help with searching promoter sequences for transcription factor Mike Holloway
DNA recovery from gels Gene Holowachuk
nuclear localization/ mammalian vector Peter Horn
Home-Made TA cloning Robert Horton
pT7-7 cloning Robert Horton
DNA sticking to glass Robert Horton
Reverse transcriptase efficiency? Eivind Hovig
RDA Mike Hubank
nuclear vs. total RNA isolation question Martin Hughes
help needed about western blots Martin Hughes
fungi in the incubator Martin Hughes
Need Info on Murine Polyoma Virus A3 Martin Hughes
Request help with p53 Martin Hughes
sequencing Martin Hughes
Plasmid loss after restreaking Martin Hughes
GETTING RID OF EtBr Martin Hughes
Req: Northerns/Chemiluminescence Gary Hunnicutt
B/W Photos of Westerns ? Jim Hutchins
exposing tritium gels Jim Hutchins
Help needed in screening libraries. Jim Hutchins
Photographing gels Jim Hutchins
DNA purification from agarose gels Keith Hutchison
OD600/cell strain relationships Robert Hutton
Best way to get DNA from gel bands? Robert Hutton
pcr primer selection shareware or freeware? Soo-in Hwang
GFP expression in mammalian cells Ildo Nicoletti, MD
Web Page Announcement Scott Ireland
5'-RACE J.E.Pease
Hydrophobic protein purification JSIGAFOOS
Hydrophobic protein purification JSIGAFOOS
Hydrophobic Peptide: Fragment Detection JSIGAFOOS
Q on Antisense-Oligo Design Jack M. Bernstein, M.D.
Antisense oligo experiments - help Jack M. Bernstein, M.D.
fungi in the incubator Jack M. Bernstein, M.D.
formylation of tRNA-fmet Narasimham Jammi
commercial Yeast genomic DNA Raymond B. Jansen
Ligation in 5 min! Changcun Jia
IRES Changcun Jia
nuclear run on --- help Changcun Jia
luciferase activity's photon counting imaging Changcun Jia
luciferase assay Changcun Jia
cosmid stability William Jia
bcl-2 antibody William Jia
re ICI products John Brennand, Tel 4639/5739/6636
re ICI seeds John Brennand, Tel 4639/5739/6636
K562 Transfection Candy Johnson
T7 expression vectors Dan Johnson
Help with searching promoter sequences for transcription factor recognition sequences David Johnson
In vitro transcription, Ambion's secret? Jillian Johnston
DNA aligning software? Jonathan Kurtis, Ph.D.
Artefacts on SDS PA gels Malcolm Moos Jr.
Does RNA - PCR ?? KRISTIN
DNA purification from agarose gels Makoto Kamei
Home-Made TA cloning Makoto Kamei
Direct PCR from E. coli colonies David Karaolis
In-situ PCR compared with in-situ hybridization Frank Karlsen
direct sequencing Suzuki Kazuhiko
direct sequencing Suzuki Kazuhiko
PLEASE HELP: > GENE CONSTRUCTS < Gabre' Kemp
SOB Medium: Why add Mg late? Nigel Kenward
SV40, large T antigen and transformed cells??? Nigel Kenward
Yeasy PCR screening problem Nigel Kenward
Sma I religation problem Nigel Kenward
DNA sticking to glass Nigel Kenward
Reusing "disposable" electroporation cuvettes Nigel Kenward
Northern blotting Nigel Kenward
NeoR gene expression from expression vectors ? Nigel Kenward
Mac software for plasmid maps Nigel Kenward
Tunicamycin & COS7 cells Nigel Kenward
HELP!! - ExoIII-mung bean digests Michael Kertesz
Useful Web Pages for MolBiologists Chong-Yee Khoo
Home-Made TA cloning Daniel Kim
B/W Photos of Westerns ? Daniel Kim
RNA gels - staining and denaturing Daniel Kim
Chemiluminescent substrate for alk-phos? Daniel Kim
Help: Blotting DNA from acrylamide gel Daniel Kim
random Mutagenesis Daniel Kim
HELP -DNA extraction from phage Daniel Kim
Preparation of Cell surface antigen Dooyeon Kim
Preparation of Cell Surface Antigen Dooyeon Kim
protease isolation? Hanbok Kim
split in dried gel? Hanbok Kim
how to make skim milk soluble? Hanbok Kim
seemingly two bands of one band in SDS-PAGE? Hanbok Kim
Sequencing from cDNA Nameer B. Kirma
Backgroud Quencher Sohei Kitazawa
Sequence Homology Software Richard Klemm
Coomassie related question Dima Klenchin
Coomassie related question Dima Klenchin
Mammalian Electroporation?!? Dima Klenchin
silver staining Dima Klenchin
Reusing "disposable" electroporation cuvettes Dima Klenchin
transfect Dima Klenchin
transfect Dima Klenchin
Artefacts on SDS PA gels Dima Klenchin
DMSO in CaCl2 Competent Cells Dima Klenchin
anti c-myc antibody needed? Dima Klenchin
?He: gelatin block for Westerns? Dima Klenchin
SDS-PAGE problem! Dima Klenchin
Rapid delivery of Lectins ?! Dima Klenchin
Immunoblot sensitivity Dima Klenchin
how about bionet.molbio.pcr? Dima Klenchin
Immunoblot sensitivity Dima Klenchin
bcl-2 antibody Dima Klenchin
Homemade ABC kit Dima Klenchin
Address/phone needed for Genex Corp. Dima Klenchin
gel drying Dima Klenchin
CTAB: How to crystallize? Knepper, Marc A
MicroProbe Corporation? Knepper, Marc A
Custom DNA sequencing Rinkei Ko
Gram positive expression vector Rinkei Ko
intracellular action of terfenadine Sei Kobayashi
F1 endonuclease? Eric Kofoid
Sensitive technique for Ang II ? Val Kolpakov
SIGMA address Prachya Kongtawelert
ethylation interference John Korte
How to import MD PhosphorImager images? Jarkko Kortesmaa
how about bionet.molbio.pcr? Jarkko Kortesmaa
Accurate and Long PCR Sasha Kraev
the missing link Jeff Kramer
Looking for a computer program Andy Kravetz
DNA Strider for PCs Andy Kravetz
Looking for HSP Antibodies Tobias Kuhme
Why probe hybridizes to vector? Peter Kuhnert
GFP expression in mammalian cells Stuart Kuhstoss
Need lacZ gene to be used as a promoter probe reporter martin LEACH
Merck Index on the internet? martin LEACH
Merck Index on the internet? martin LEACH
Pharmacia UK email address? martin LEACH
E-mail address of INVITROGEN martin LEACH
Electrophoresis newsgroup? martin LEACH
transfect martin LEACH
Info on comercial RNA synthesis need. martin LEACH
Home-Made TA cloning martin LEACH
SOURCE OF LUCIFERASE martin LEACH
A Source of Company email/WWW addresses martin LEACH
Tm calculation and software martin LEACH
How to control the PCR where genomic DNA martin LEACH
Home-Made TA cloning martin LEACH
Stability of Luc or GFP Protein martin LEACH
To NEST GROUP martin LEACH
luciferase assay martin LEACH
P1 cloning LI, TANG
p1 cloning system LI, TANG
tel or e-mail for S.M.Jazwinski ULSMC LOGAN
Rf: Plasmid sequencing: Methods to improve resolution LOGAND
Rf[2]: Plasmid sequencing: Methods to improve resolution LOGAND
Rf: Sequencing from cDNA LOGAND
Yeast DNA fingerpinting McDonald Lab
Sequencing Problem The Wagner Lab
I lost the inverse PCR article! John Ladasky
Artefacts on SDS PA gels Mark Lamkin
FGF Sources Carlisle Landel
cauliflower mosaic virus promoter Bill Langridge
Commercial source for Two-hybrid libraries? Michelle Larsen
Commercial source for Cre? Michelle Larsen
RNA-prep from starch-rich tissue Bernard Lassegue
PCR Mutagenesis Bernard Lassegue
Photographing gels Wolfgang Latz
SOB Medium: Why add Mg late? Jeff Lawrence
Plasmid sequencing: Methods to improve resolution Jeff Lawrence
A549 lung alveolar carcinoma Denis LeBel
To NEST GROUP Martin Leach
picking degenerate PCR primers Kevin Lease
PCR of lambda gt10 Kevin Lease
Rap-PCR Stephen P Lee
Stripping Hybond membranes Rachelle Leger
TAQ Question Rachelle Leger
PCR methods?? V Lekhakul
HepG2 nuclear extract Rainer Lemke
B/W Photos of Westerns ? Jeff Leonard
Ultra-low freezer recommendation LindaG7202
test Tsu-Hwie Liu
PCR on fresh plant tissue? Tsu-Hwie Liu
FITC&receptor binding Mark Logan
chromosome 15 Benke/Belmar Login
transfect Benke/Belmar Login
library' Benke/Belmar Login
cDNA library source? Benke/Belmar Login
cDNA library Benke/Belmar Login
Sequencing gel solution premixes Billy B. Long
sequencing Billy B. Long
PCR to detect viral point mutations Francesc X Lopez-Labrador
Who sells Gilson pipettors? Ted M.
3kB PCR Cycling Conditions?// Ted M.
Home-Made TA cloning Ted M.
ABI DNA Software 2.1.0/PowerMac/HP1200C Ted M.
PCR in ten minutes! Ted M.
inclusion bodies Ted M.
how about bionet.molbio.pcr? Ted M.
inclusion bodies Ted M.
GETTING RID OF EtBr Ted M.
HELP: inconsistent genomic PCR troubleshooting Ted M.
San Diego Molecular Biology Conf MICROPLATE
LOOKING FOR COLABORATIONS FOR NEW MAGNETIC POROUS GLASS TECNOLOGY MICROPLATE
how about bionet.molbio.pcr? MICROPLATE
how about bionet.molbio.pcr? MICROPLATE
unsubscribe MPOLAK at CRCVMS.UNL.EDU
Seeking for a source of Nitrocefin Juan MacFarlane
Tth111I Emma Macfarlane
RDA Amanda Mackie
A good method for nested deletion mutants? S. J. Mc Mahon
hekp with sigmacoat and sequencing gels Jeffrey Mahr
help X-gal Stephen Mamber
How many E. coli = 1 O.D. unit? Stephen Mamber
DMSO in CaCl2 Competent Cells Stephen Mamber
Need ideas for Lac+/- screening in Neisseria Stephen Mamber
how to make skim milk soluble? Stephen Mamber
Induction by IPTG Stephen Mamber
NIH Grant Forms Richard Mandel
Why probe hybridizes to vector? Amy Marion
RNA gels - staining and denaturing Amy Marion
RNA probes Stewart G Martin
Sealing GFP coverslips Dr S T May
48 cm WTR runs on ABI 377s George F. Mayhew
Glucose/Lactate analyzers - suppliers? Mays, Peter
gelatin block for Westerns Helen McBride
beta-ME in transformations? Tara McCready
his-tagged protein purification with metal chelate columns Tara McCready
Calculating Tm - Help Needed Nigel McMillan
GST-Fusion proteins Frances Mcfarland
B/W Photos of Westerns ? Scott Selby Med-Hem
SOB Medium: Why add Mg late? Pierre-Alain Menoud
Help needed with in situ hybrisiation Pierre-Alain Menoud
Direct PCR from E. coli colonies Pierre-Alain Menoud
silver staining Pierre-Alain Menoud
Source for Radiolabeled G418 Kojo Mensa-Wilmot
DNA Chips definition Patrick Merel
HumARA ladder Patrick Merel
Ligations-HELP David Micklem
Ligation in 5 min! David Micklem
DNA purification from agarose gels David Micklem
Ligation problem Mikek
fungi in the incubator Andrey Mikhailow
IPTG and induction, new info Kenneth Minneman
LPS protein Len Moaven
5'-RACE Mohanish at pharmdec.wustl.edu
Sma I religation problem Barry Moore
ABI Sequence Signal Weak WHY!? Sean David Moore
PCR Problem Grant Morahan
Designing PCR primers-How to check them? Grant Morahan
In vitro transcription, Ambion's secret? Michael Morales
Southern-PCR Shahram Mori
HPLC of L-amino acids Andrea Moriondo
RNA gels - staining and denaturing Andrea Moriondo
protein concetration Alex Morla
mapping the transcriptional start site: HELP!! Heidi Moss
SOURCE OF LUCIFERASE T. J. Murphy
Alternative to Zeocin? Bernard Murray
Wanted: Anti-LacI or HSV-VP16 Bernard Murray
Address/phone needed for Genex Corp. Bernard Murray
Address/phone needed for Genex Corp. Bernard Murray
Address/phone needed for Genex Corp. Bernard Murray
Luciferase/CAT assay cell extracts? Bernard Murray
p1 cloning system Michael Myers
[Q] How to purify small PCR frg. ? Michael Myers
Best way to get DNA from gel bands? Michael Myers
Rf: Sequencing from cDNA Michael Myers
Calculating Tm - Help Needed Peter Myler
research of an article in DNA Seq. Journal Myriam
Direct PCR from E. coli colonies Daniel Mytelka
inclusion bodies Daniel Mytelka
Photographing gels CK NG
DNA recovery from gels Jon Nakamoto
molecular weight of oligos Your Name
PCR GC-rich genomic DNA Nancy J. Phillips, M.D.
Need RIA for mouse hormones Francois Nantel
Temp of Dry ice? (ie sublimation point) Francois Nantel
Palindrome and ligations Francois Nantel
Mysterious probes do not bind southern Francois Nantel
Direct PCR from E. coli colonies John Nash
The half-life of CAT mRNAs and proteins Alain Nepveu
Northern blotting Alain Nepveu
Statistics for biologists. Rick Neubig
Hydrophobic Peptide: Fragment Detection Rick Neubig
cleaning up IEF tubes? John A. Newitt
[Q] L-amino acid kits John A. Newitt
disulphide location in short peptides John A. Newitt
Hydrophobic Peptide: Fragment Detection John A. Newitt
Proper Attribution (was Re: Hydrophobic Peptide: Fragment detection John A. Newitt
Looking for Clostridium McAb Usenet News
Call for LacZ fragment for terminal bash Brad Nicholson
Help! - EMS mutagenesis Brad Nicholson
Direct PCR from E. coli colonies (GENOMIC???) Brad Nicholson
cosmid stability Brad Nicholson
E coli expression of eucaryotic protein Brad Nicholson
rabbit-elisa-assays To: Nikolai
none.(separation T and B) To: Nikolai
detection of disulphide bridges? To: Nikolai
silver staining To: Nikolai
sequencing To: Nikolai
Purification of 6-His tagged proteins on Ni-NTA To: Nikolai
SIGMA address To: Nikolai
How to import MD PhosphorImager images? Andy Law Big Nose
Who sells Gilson pipettors? Andy Law Big Nose
Does RNA - PCR ?? Andy Law Big Nose
fungi in the incubator Andy Law Big Nose
384 pin tool for biomek 1000 Andy Law Big Nose
PCR to detect viral point mutations Kevin O'Donnell
Ligation in 5 min! Guy Oshiro
Ligation in 5 min! Guy Oshiro
WWW site about Molec Biol and Biocomputing Peter Van Osta
WWW address for John Innes Center Francis Ouellette
Help with mitochondria P.Stening
What is retrotransposon? BANANA & PLANTAIN
5-FOA solubility AL R. PLUMMER
help: cloning oligos Soon Paik
cytomegalovirus promoter Soon Paik
pcr primer selection shareware or freeware? Soon Paik
pcr primer selection shareware or freeware? Soon Paik
electroporation of BL21(DE3)pLysS Nadesalingam N Palaniyar
detection of disulphide bridges? Mark Pallen
WANTED: biologists' opinions on Internet information sources Mark Palmer
[Q] How to purify small PCR frg. ? Julian Parkhill
Any BIO-RAD email, gopher, or WWW address? Morris Paterson
Glass Beads Patricia
cDNA from total RNA? Patricia
Total DNA Preparation Poon Kwong Yiu Patrick
RNA gels - staining and denaturing Aaron Pawlyk
exposing tritium gels Jeffrey Pepe
Mammalian Episomal Vectors/B-gal Neil Percy
Sequencing Problem Luiz Filipe F Pereira
Intron Mapping Luiz Filipe F Pereira
his-tagged protein purification with metal chelate columns Peter
Designing PCR primers-How to check them? Peter---bkxo at musicb.mcgill.ca
Users of BIO-RAD Molecular Imager Systems... Howard T. Petrie
DNA extraction from fungal mycelia Rodney Earl Pettway
Yeast DNA fringerprinting Rodney Earl Pettway
Coomassie related question Stephen P. Driska PhD
Does size matter? dsDNA IS probes Robert Pierce
DNA aligning software? Robert Pierce
Epitope Mapping Kits: Chiron Mimotopes vs. Genosys Spots Kit? Seth Pincus
gp-120 participant has questions... Seth Pincus
GST fusion prots truncating at 27 kDa? Jeff Pitman
KODAK LPD4 Gopi K. Podila
GETTING RID OF EtBr Steven Poet
GETTING RID OF EtBr Steven Poet
Eucaryotic expression vector for tagged protein. Philippe Pognonec
Sma I religation problem Mike Poidinger
Anyone have any GOOD FTP/WEB sites for Biology Freeware/Shareware Mike Poidinger
5-FOA solubility Scott Vande Pol
lacZ reporters Scott Vande Pol
yeast/mammalian expression plasmids Scott Vande Pol
e.coli strain dnaB ts Oliver Politz
Help in SDS-PAGE Constantin Polychronakos
how to remove albumin Andrei Popov
5-FOA solubility Andrei Popov
Home-Made TA cloning Andrei Popov
Sma I religation Andrei Popov
YAC transfer Andrei Popov
fungi in the incubator Andrei Popov
cosmid stability Andrei Popov
Immunoblot sensitivity Andrei Popov
Cosmid Transformation Ned Powell
Sequencing gel solution premixes Eddie Power
silicon carbide whisker C. S. Prakash
Source of oligos for repeats in plants C. S. Prakash
Desperately Seeking pRSET QIAGEN
DNA purification of lambda gt11 clones QIAGEN
harvesting DNA from a lambda gt11 clone QIAGEN
[Q] How to purify small PCR frg. ? QIAGEN
E coli expression of eucaryotic protein QIAGEN
Best way to get DNA from gel bands? QIAGEN
Difficult DNA - Ref Needed The Ice Queen
laying membranes on bacteria plates R.D.Johnson
Why probe hybridizes to vector? R.D.Johnson
Why probe hybridizes to vector? R.D.Johnson
H. pylori secreted protein antibodies? R.D.Johnson
Gel printer/photodoc system TRACEY ANN RISTOW
genomic DNA isolation RMCMAH93 at IRLEARN.UCD.IE
Info needed on SUBTRACTIVE HYBRIDIZATION Berend Rah
Somatic in-vivo adenovirus gene therapy Berend Rah
Need Urgent Help ! PCR DIFFERENTIAL DISPLAY ! Berend Rah
Need email of Invitrogen Berend Rah
Fluorimager Mukul Ranjan
Sma I religation problem Colin Rasmussen
Sequencing gel solution premixes Colin Rasmussen
hekp with sigmacoat and sequencing gels Colin Rasmussen
5'-RACE Soren K. Rasmussen
Why probe hybridizes to vector? Marko Rehn
Aby to RBC Ghost Protein? Tanya Reichert
Protocol needed stain Cos cells for DNA,RNA Mary P. Remington
in vitro tranlation of retroviral gene Mary P. Remington
Non-radioactive Southerns and northerns-help Mary P. Remington
pcDNA3 in BHK-cells Dietmar Rescheleit
Sequencing PCR products Immune Response
Schleicher & Schuell,Inc phone number?????? Immune Response
AZLON BAGS Michael Rhodes
Waffle iron PCR machine Michael Rhodes
(no subject) Michael Rhodes
Direct PCR from E. coli colonies (GENOMIC???) Richard_Heath
exposing tritium gels Richard_Heath
Purifying Bacto-Agar: Suggestions? Todd Richmond
Reusing "disposable" electroporation cuvettes Todd Richmond
SIGMA address Brad Riesenberger
E. coli transformation Stéphane Rioux
(no subject) Elizabeth Robinson
Hamster cDNA or genomic libraries? Elizabeth Robinson
Ion-exchange Chromatography with Enzymes Rod
PCR problem - please help Richard M Rohan
How to remove albumin? Rory.Morty
Help: COncanavalin A chromatography Rory.Morty
searching for keratinocyte cDNA library Pamela A. Rose
y Pamela A. Rose
searching for keratinocyte cDNA library Pamela A. Rose
searching for keratinocyte cDNA library Pamela A. Rose
Statistics for biologists. Elizabeth Rosenberg
UK phone no. for Bio/Gene Colin Ross
Reusing "disposable" electroporation cuvettes Ian Ross
RNA gels - staining and denaturing Ian Ross
Protein elution from Ab columns Ian Ross
Artefacts on SDS PA gels Ian Ross
Who knows the tricks for S1 or primer Ext Traci Roth
KODAK LPD4 Hiranya Roychowdhury
klenow Hiranya Roychowdhury
cleaning up IEF tubes? Hiranya Roychowdhury
Coomassie related question Hiranya Roychowdhury
DNA recovery from gels Hiranya Roychowdhury
molecular weight of oligos Hiranya Roychowdhury
Help: Blotting DNA from acrylamide gel Hiranya Roychowdhury
Any BIO-RAD email, gopher, or WWW address? Hiranya Roychowdhury
GST-Fusion proteins Hiranya Roychowdhury
exposing tritium gels Hiranya Roychowdhury
SDS-PAGE problem! Hiranya Roychowdhury
Lambda Hind III Marker Does anyone know of a cheap source for Lambda Hind III? Paying over $100 for 500 ug seems a little steep. Hiranya Roychowdhury
Reusing "disposable" electroporation cuvettes Hiranya Roychowdhury
SDS-PAGE problem! Hiranya Roychowdhury
how about bionet.molbio.pcr? Hiranya Roychowdhury
Best way to get DNA from gel bands? Hiranya Roychowdhury
<None> Hiranya Roychowdhury
[Q] How to purify small PCR frg. ? Peter Rudolph
gp-120 participant has questions... Ray Russ
Yeast (S.c.) RNA pol II terminator? Peter Russell
Current Contents phone# Doug-young Ryu
Plasmid sequencing: Methods to improve resolution S941407 at mailserv.cuhk.hk
E-mail address of INVITROGEN S941407 at mailserv.cuhk.hk
PCR vs Plasmid sequencing S941407 at mailserv.cuhk.hk
Designing PCR primers-How to check them? SEHuang
Does RNA - PCR ?? SEHuang
Commercial Plasmids Comment Somphob Leetachewa - ST
Where are codon pref. tables? Klaus Salger
Chemiluminescent substrate for alk-phos? Klaus Salger
info on GenSys (?) automated sequencer Tony Sanchez
info on Scotlabs Tony Sanchez
ALF Woes! Ari-Matti Sar‚n
Stripping Hybond membranes Robert D. C. Saunders
4-thioUTP reactivity Brennan S Sawyer
San Diego Molecular Biology Conf Karim Sayani
Ligation in 5 min! Karim Sayani
DNA amplification Larry Scaramella
Avanti Polar Lipids ? Wolfgang Schechinger
Electrophoresis newsgroup? Wolfgang Schechinger
Q on Antisense-Oligo Design Wolfgang Schechinger
Antisense oligo experiments - help Wolfgang Schechinger
Calculating Tm - Help Needed Wolfgang Schechinger
Info on comercial RNA synthesis need. Wolfgang Schechinger
Ion-exchange Chromatography with Enzymes Wolfgang Schechinger
exposing tritium gels Wolfgang Schechinger
ATCC book on freeze drying methods Wolfgang Schechinger
how about bionet.molbio.pcr? Wolfgang Schechinger
<None> Wolfgang Schechinger
Electrophoresis Scientist -want a free trip? Wolfgang Schechinger
GETTING RID OF EtBr Wolfgang Schechinger
GETTING RID OF EtBr Wolfgang Schechinger
info on chemicals Wolfgang Schechinger
GETTING RID OF EtBr Wolfgang Schechinger
Synchronizing Mammalian Cells? Anyone tried this?! Andrews Schrader
Isol. of Transcription factors Bernhard Schrautemeier
Transcription factors with SurfZAP? Bernhard Schrautemeier
material transfer agreements Edgar Schreiber
PCR in ten minutes! Geoff Scopes
pME240 Vector Christian Seelos
exposing tritium gels Bart Sefton
inclusion bodies Volker Seibert
DNA recovery from gels Chris Seidel
DNA purification from agarose gels Spencer Seidel
Need ideas for Lac+/- screening in Neisseria Hank Seifert
sequencing Shauna R. Brummet, Ph.d.
Avanti Polar Lipids ? Incheol Shin
Has anyone used Heraeus Instruments miniPERM? Ray Harrison Shirk
Coating LB plates with tet after pouring? Nathan O. Siemers
PCR-primers for cos-ends?? Eirikur Sigurdsson
Help needed with in situ hybrisiation Alan Silverman
DNA extraction from fungal mycelia Sim
disulphide location in short peptides Gurmil Singh s/o Dalip Singh
Lambda Hind III Marker Does anyone know of a cheap source for Lambda Hind III? Paying over $100 for 500 ug seems a little steep. Michael Ian Sklar
Coating LB plates with tet after pouring? Ole Skovgaard
Stripping Hybond membranes Jeffrey Smerage
Help: Blotting DNA from acrylamide gel Jeffrey Smerage
harvesting DNA from a lambda gt11 clone Donna Smith
What is retrotransposon? Felipe N. Soto
Immunoblot sensitivity Giorgio Spagnol
Designing oligos for PCR Giorgio Spagnol
MAGNETIC POROUS GLASS (MPG0 Giorgio Spagnol
LOOKING FOR COLABORATIONS FOR NEW MAGNETIC POROUS GLASS TECNOLOGY Giorgio Spagnol
silver staining Giorgio Spagnol
silver staining Giorgio Spagnol
[Q] How to purify small PCR frg. ? Giorgio Spagnol
SDS-PAGE help badly needed Giorgio Spagnol
Help in SDS-PAGE Giorgio Spagnol
Western help needed! Anne Spang
Lysing yeast spheroplasts Anne Spang
GST-Fusion proteins Anne Spang
?He: gelatin block for Westerns? Anne Spang
Analytichem International Ulrich Sperling
RNA gels - staining and denaturing Cathy Sprankle
Small-scale mRNA prep kits/methods Cathy Sprankle
Storage of B-gal staining buffer Matt Springer
E.mail adress to Invitrogen Anne Spurkland
HELP with glcyerol-tolerant sequencing gels SquireNige
Northern blotting SquireNige
DD-PCR KITS/Companies..... Hilary K. Srere
Pre-flashing films for ECL?? Srinivas K. Janardan, M.D.
Home-Made TA cloning Srinivas K. Janardan, M.D.
MDE-Hydrolink: where to buy?? Darren M. Stauth
Home-Made TA cloning Stephen R. Lasky, Ph.D.
RNA gels - staining and denaturing Stephen R. Lasky, Ph.D.
fungi in the incubator Stephen R. Lasky, Ph.D.
PCR primers for AP-1 complex Stephen R. Lasky, Ph.D.
exposing tritium gels Stephen R. Lasky, Ph.D.
A Source of Company email/WWW addresses Stephen R. Lasky, Ph.D.
exposing tritium gels Stephen R. Lasky, Ph.D.
RT-PCR on viral RNA Steven_Burghart at BAYLOR.EDU
Isol. of Transcription factors Sean Stevens
Transcription start site determination. Sean Stevens
PCR on fresh plant tissue? Neal Stewart
Southern Hybridization Buffers? Wendy Stewart
Urgent Help! French & Italian Electrophoresis Scientists Chris J Stone
Electrophoresis Scientist -want a free trip? Chris J Stone
Help - isolation of DNA from snake cross-sections Gregory Stuart
Plasmid sequencing: Methods to improve resolution Steven Sullivan
Need opinions on DDPCR idea Steven Sullivan
Direct PCR from E. coli colonies Steven Sullivan
DD-PCR annoyance Steven Sullivan
Does RNA - PCR ?? Steven Sullivan
RNA gels - staining and denaturing Steven Sullivan
Ligation problem Steven Sullivan
Sequencing from cDNA Steven Sullivan
immunohistochemistry protocol William Sun
rat brain cDNA libraries William Sun
rat brain cDNA library William Sun
Ligations-HELP Sailesh Surapureddi
lacZ reporters Sailesh Surapureddi
DNA recovery from gels Sailesh Surapureddi
[Q] How to purify small PCR frg. ? Sailesh Surapureddi
Help in SDS-PAGE Sailesh Surapureddi
Help needed with in situ hybrisiation Mr D J Sutherland
Method wanted:-Enhancement of AP linked in situ's with PVA Mr D J Sutherland
Help needed with in situ hybrisiation Mr D J Sutherland
Help needed with in situ hybrisiation Mr D J Sutherland
Distinguishing between Osteoblasts and Stromal Cells? Russell Taichman
How to control the PCR where genomic DNA as a template Dr GR Taylor
Storage temperatures Rick Tearle
Direct PCR from E. coli colonies Rick Tearle
PCR from blood Rick Tearle
PCR Question Christopher M. Tebeau
hekp with sigmacoat and sequencing gels Christopher M. Tebeau
DNA purification from agarose gels CMMID Va Tech
Sequencing PCR products Jeffrey Shane Temple
histone preparation Tom Thatcher
nuclear localization/ mammalian vector Tom Thatcher
Expression of GFP in eukaryotic cells. Dr. Ulrich Thoenes
GFP expression in HEPG2 cells Ulrich Thoenes
GFP expression in Fibroblasts and K46 B-cells Ulrich Thoenes
(no subject) Bruce R. Thomas
sugar beet genomic library wanted Bruce R. Thomas
gelatin block for Westerns John R. Thompson
DNA purification from agarose gels Tim
Ligation in 5 min! Tim
peptide synthesis Tim
DNA Sequencing-HELP TimHusky
Ligations-HELP TimHusky
Reusing "disposable" electroporation cuvettes Miss E.T. Tonkin
thermocyclers Walter L. Trudeau
Photographing gels John Tudor
Who sells Gilson pipettors? Michael J. Tuvin
Ligation in 5 min! Michael J. Tuvin
pT7-7 cloning Michael J. Tuvin
Ultra-low freezer recommendation Michael J. Tuvin
Northern blotting Michael J. Tuvin
(none) Michael J. Tuvin
Thermocyclers Michael J. Tuvin
MoAb fragmentation using ficin U12201 at uicvm.uic.edu
DNA & Microtiter Plates U12201 at uicvm.uic.edu
Any BIO-RAD email, gopher, or WWW address? U12201 at uicvm.uic.edu
DMSO in CaCl2 Competent Cells U12201 at uicvm.uic.edu
Rapid delivery of Lectins ?! U12201 at uicvm.uic.edu
gelatin block for Westerns U12201 at uicvm.uic.edu
Catalogs: VWR,SarWel,Grainger,McMaster,LabLine.. Vasos Panagiotopoulos +1-212-287-8087 Bioengineer-Financier Samani Marions Panyaught NYC 11357-3436-287 USA
In vitro transcription, Ambion's secret? Paul E. Ulanch
What is retrotransposon? Paul E. Ulanch
Thermocyclers Karen Usdin
Thermocyclers Karen Usdin
monoclonal Abs in bacteria User
MAbs in Bacteria User
human rDNA's request Antoine VEKRIS
mtDNA molluscs primers? Javier Quintero Vazquez
Help on transfecting JURKAT! Javier Quintero Vazquez
Cell Attachment to culture flasks. Arthur Vickers
protein concetration Marc Visconti
(no subject) Visu
random Mutagenesis Visu
PCR in ten minutes! Richard Vogt
PCR in ten minutes! Richard Vogt
Optimal conditions for PCR of small inserts Richard Vogt
Amersham ECL components? NIGEL JOHN WALKER
sequencing gel solution premixes WANDERS at INDYVAX.IUPUI.EDU
!!*NEW Experimental Medicine WEB Page*!! WEBMASTER
RAPD Gel Analysis ANTHONY WILSON
RAPD Problems - Control lanes with bands ANTHONY WILSON
UV Sterilization ANTHONY WILSON
test Helen E. WIlson
Help - RNA isolation Helen E. WIlson
P24/CD9 western antibody! WZhao80850
SDS-PAGE problem! WZhao80850
Who knows the tricks for S1 or primer Ext Dr. Kimberly Walker
Still Looking for antiB-Glucuronidase antibodies Joseph Walker
Looking for anti B-Glucuronidase antibodies Joseph Walker
Amersham ECL components? Nigel Walker
Sequencing from cDNA Nigel Walker
Best way to get DNA from gel bands? Nigel Walker
dna prep for eukaryotic cells (macrophages)? Don A. Walthers
Chemiluminescent substrate for alk-phos? Don A. Walthers
PCR cloning Edward Wang
Calculating Tm - Help Needed Shiao Y. Wang
Calculating Tm - Help Needed Shiao Y. Wang
Difficult DNA - Ref Needed Shiao Y. Wang
Lysing yeast spheroplasts Julie Wasylnka
efficient blunt ligation? Matthew J. F. Waterman
hekp with sigmacoat and sequencing gels Matthew J. F. Waterman
Plasmid prep'ing machine A. John Watson
Southern Hybridization Buffers? A. John Watson
Taq polymerase from Fisher Dan Wells
Why probe hybridizes to vector? Wenfu
Mycoplasma Detection Johnny Wharton
Apa1 digestion Philippa Whitelaw
DNA & Microtiter Plates Roger Wiegand
hekp with sigmacoat and sequencing gels Don Wigington
Help needed with FISH Gareth Williams
Address/phone needed for Genex Corp. Paul Wingfield
how about bionet.molbio.pcr? Harry Witchel
Mysterious probes do not bind southern Harry Witchel
2HybridSystem: Gal4 or LexA Stephan Witte
[Q] How to purify small PCR frg. ? Patricia Wolff
DNA Strider for PCs Jim Woodgett
immobilizing antibodies, peptides and other biomolecules John Woodward
Direct PCR from E. coli colonies William D. Woolfolk
[Q] L-amino acid kits Mayumi Yagi
Calculating Tm - Help Needed Mayumi Yagi
HELP -DNA extraction from phage Mayumi Yagi
Sequencing from cDNA Ann M. Yezerski
DNA aligning software? Ann M. Yezerski
Yeasy PCR screening problem Yi
pCDNAI and pCDM8 problems in stable transfection ? Ian A. York
Expression cloning in COS Ian A. York
unsubscribe methods-and-reagents Yoshiyuki Yoshida
Home-Made TA cloning Youre-mailaddress
hekp with sigmacoat and sequencing gels Youre-mailaddress
how to make skim milk soluble? Youre-mailaddress
RAP-PCR David Yu
PCR primers for AP-1 complex David Yu
Lysing yeast spheroplasts Dan Zabetakis
Aberrant Crypts ZipHelms
In vitro transcription, Ambion's secret? Frances Hannan Zoology
Help: Blotting DNA from acrylamide gel Frances Hannan Zoology
Glycerol loading buffer in acrylamide gels? Zorg
E. coli B834 cells Peter Zwickl
(no subject) Peter Zwickl
Phospholipase D assay acdumaua at indyvax.iupui.edu
polyA mRNA Isolations. albert
DNA & Microtiter Plates allang at cts.com
DNA extraction from Tobacco/Petunia anon at lamar.colostate.edu
immunohistochemistry in human duodenum anonymous
protein fractionation ar at rosenthal.com
Source for TIMPs and PAIs ? arturo.galvani at itner.pharmacia.se
DMSO in CaCl2 Competent Cells arturo.galvani at itner.pharmacia.se
SDS-PAGE problem! arturo.galvani at itner.pharmacia.se
split in dried gel? b574713
how leaky is it for lacIq? benedik at uh.edu
Role of deoR and plasmids benedik at uh.edu
Coomassie related question dinakar bhattramakki
RNA gels - staining and denaturing dinakar bhattramakki
DNA purification of lambda gt11 clones dinakar bhattramakki
Looking for HSP Antibodies dinakar bhattramakki
Plasmid loss after restreaking dinakar bhattramakki
DNA recovery from gels bicfo at flinders.edu.au
DNA Sequencing-HELP bio.tamu.edu
Gel Purification by syringe method joe blow
HELP: inconsistent genomic PCR troubleshooting bmay
HELP: inconsistent PCT troubleshooting bmay
microtiterplates for sequencing bmay
Designing PCR primers-How to check them? brett
Apa1 digestion brett
Promoter Finder Kit from Clontech phillip buckhaults
How to import MD PhosphorImager images? phillip buckhaults
Ligation in 5 min! phillip buckhaults
pBeloBac II bweil at mzdmza.zdv.uni-mainz.de
pUC18/19 double digest with BamHI/SmaI bweil at mzdmza.zdv.uni-mainz.de
DNA purification from agarose gels andy chien
PCR of lambda gt10 chihara
PCR of lambda gt10 chihara
PCR WWW.site quin chou
Tm calculation and software quin chou
Merck Index on the internet? cjackson
Where can I find FAQ? CCSO Sites Services client
neo primer/genomic southern question! converrl at ucbeh.san.uc.edu
efficient blunt ligation? hong dang
Info on comercial RNA synthesis need. hong dang
campylobacter vectors. please!,, iain andrew davidson
RNA oligos ? diw at mail.nerc-oxford.ac.uk
Cell Culture - How to isolate membranes ? f.r.byrne
Why probe hybridizes to vector? faerber at ubaclu.unibas.ch
Why probe hybridizes to vector? faerber at ubaclu.unibas.ch
Why probe hybridizes to vector? gaterman at mzdmza.zdv.uni-mainz.de
PCR Mutagenesis neill gingles
PCR from blood neill gingles
DNA recovery from gels giorgio
silver staining giorgio
dye for cells giorgio
How to control the PCR where genomic DNA as a template giorgio
RNA-prep from starch-rich tissue grohmann at mpimg-berlin-dahlem.mpg.de
internal probe for Ara Northern blots grohmann at mpimg-berlin-dahlem.mpg.de
glycerol effect with TBE; RE: gsbs1018 at UTSPH.SPH.UTH.TMC.EDU
Need Info on Murine Polyoma Virus A3 gvacano at gems.vcu.edu
In vitro transcription, Ambion's secret? jim hartley
Home-Made TA cloning jim hartley
just a test jacqueline heard
HELP -DNA extraction from phage jacqueline heard
HELP: Why the PAGE gel cracks sometimes? huangbx at DEAKIN.EDU.AU
Mac software for plasmid maps jeg at PW.USDA.GOV
bovine genomic library wanted jfh
3' RACE - looking for suggestions jfh
Sequencing gel solution premixes jfh
PCR of lambda gt10 jfh
REQUEST cDNA library from rat fibroblasts jkenny
Statistics for biologists. jon
antibodies prep. from inclusion bodies jpl at ibcp.fr
looking for lung cDNA libraries jthrppel at vax1.tcd.ie
lung cDNA libraries jthrppel at vax1.tcd.ie
B3\``(DC``").P` judi_heitz at stratagene.com
PCR Problem julia
analysis of benzoate ?? jylee
Lafmid BA vector??? k.d.preuss
Lafmid BA vector??? k.d.preuss
Lafmid BA vector??? k.d.preuss
How to remove albumin? kal at amc.uva.nl
how to work out curvature of DNA??? t. j. kappock
cosmid stability brian kent
Home-Made TA cloning Tjian lab
Southern Hybridization Buffers? Tjian lab
Silver staining laigle
silicon carbide whisker lees at SASK.USASK.CA
WWW address for John Innes Center lees at SASK.USASK.CA
ICI Seeds address lees at SASK.USASK.CA
address request lees at SASK.USASK.CA
Timber Press, Inc. lees at SASK.USASK.CA
Intelligenetics lees at SASK.USASK.CA
35S promoter patent lees at SASK.USASK.CA
info on chemicals lees at SASK.USASK.CA
SIGMA address lees at SASK.USASK.CA
mRNA purification kit lmc at sevax1
Direct PCR from E. coli colonies lmc at sevax2
Bionet USENET Articles (Search GOPHER) lonnie
WANTED: HELP COATING <uM PARTICLES lonnie
nuclear vs. total RNA isolation question lsalati at wvnvms.wvnet.edu
gel drying lw75
Protocol needed stain Cos cells for DNA,RNA m-brazil at nimr.mrc.ac.uk
Sourses for Staphy. Entero. B toxoid ? manisha at umich.edu
pCDNAI and pCDM8 problems in stable transfection ? marcvdc at lmb1.rug.ac.be
RABBIT POX VIRUS DNA marcvdc at lmb1.rug.ac.be
SWINE POX VIRUS DNA marcvdc at lmb1.rug.ac.be
hekp with sigmacoat and sequencing gels mcmanusj at omrf
Ultra-low freezer recommendation mjb1 at Lehigh.EDU
LOOKING FOR COLABORATIONS FOR NEW MAGNETIC POROUS GLASS TECNOLOGY mmccorm at macc.wisc.edu
How to control the PCR where genomic DNA mmccorm at macc.wisc.edu
immobilizing antibodies, peptides and oth mmccorm at macc.wisc.edu
Sequencing gel solution premixes msautel at ulys.unil.ch
Labelled proteins on ABI sequencer? mwatson at uniwa.uwa.edu.au
<None> mwgaunt at molbiol.ox.ac.uk
Urgent Help! French & Italian Electrophoresis Scient - WARNING mwgaunt at molbiol.ox.ac.uk
how leaky is it for lacIq? Ing nang
Suggestions needed for PCR primers for mouse X and Y chromosomes. veronica narvaez
Southern Hybridization Buffers? veronica narvaez
Calculating Tm for mismatch on Northern neale at mbcf.stjude.org
Home-Made TA cloning nsaunders at molbiol.ox.ac.uk
DNA purification of lambda gt11 clones patricia ohana
harvesting DNA from a lambda gt11 clone patricia
Help! Graduate student needs metal inducible eukaryotic expression vector plonksg at ctrvax.vanderbilt.edu
Sequence Homology Software powelld at irl.cri.nz
Seed company Re: lees@sask.usask.ca pyw
Seed company Re: lees@sask.usask.ca pyw
fungi in the incubator rand777 at ids.net
<none> rcha244 at bay.cc.kcl.ac.uk
separation of T and B lymphoctyes rcha244 at bay.cc.kcl.ac.uk
PCR artifacts rcha244 at bay.cc.kcl.ac.uk
test, please ignore rhubner at molbiol.ox.ac.uk
Does RNA - PCR ?? rmoldwin at midway.uchicago.edu
inducible mammalian expression vectors rmoldwin at midway.uchicago.edu
Home-Made TA cloning s060965 at AIX1.UOTTAWA.CA
test sabine.hanelt at medizin.uni-ulm.de
test sabine.hanelt at medizin.uni-ulm.de
32P-postlabeling sabine.hanelt at medizin.uni-ulm.de
address needed M.Schoor sabine.hanelt at medizin.uni-ulm.de
Hybridizing On NC vs. Nylon schoenfeld at MSSCC.MED.UTAH.EDU
test - please ignore! sievert at molbio.mu-luebeck.de
Schleicher & Schuell,Inc phone number?????? sjgouldrulesmiggy
Thank you all very much(was Schleicher & Schuell,Inc phone number) sjgouldrulesmiggy
Are you from Baylor University?? sjgouldrulesmiggy
Urgent Help! French & Italian Electrophoresis Scientists somebody
Where are codon pref. tables? Braudel Maqueira soporte
histone preparation steinhof at ???.Uni-Marburg.DE
To NEST GROUP cynthia stryker
Calculating Tm - Help Needed sugarB
Need lacZ gene to be used as a promoter probe reporter taylor
pTTQ18 thibauls at galaxy.ucr.edu
Northern blotting v115t2vq at ubvms.cc.buffalo.edu
fecal sample DNA varyc.mmcri at mmc.org
parvovirus PCR pos. control varyc.mmcri at mmc.org
Genomic prep for bacteria varyc.mmcri at mmc.org
klenow vdrover at kean.ucs.mun.ca
Southern-PCR vdrover at kean.ucs.mun.ca
trout nuclei isolation vdrover at kean.ucs.mun.ca
Expression of GFP in eukaryotic cells. villartay at citi2.fr
Expression of GFP in eukaryotic cells. villartay at citi2.fr
Does RNA - PCR ?? walter
PCR Problem walter
RT-PCR on viral RNA walter
RT-PCR on viral RNA walter
RT-PCR on viral RNA walter
RT-PCR and gels walter
ignore...testing pete wilson
DNA purification from agarose gels xunfang at wvnvms.wvnet.edu
nondenaturing probe in footprinting xunfang at wvnvms.wvnet.edu
(none) yskim at CAMD2.KKPCR.RE.KR
In vitro transcription, Ambion's secret? zinc

Last message date: Mon Jul 31 23:25:01 EST 1995
Archived on: Sun Mar 6 20:39:42 EST 2005

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