labmda terminase

Jose Casal Lince jec1 at mrc-lmb.cam.ac.uk
Thu Jun 1 06:40:34 EST 1995


In Article <3qj81t$176s at news.doit.wisc.edu>, smango at macc.wisc.edu (Susan
Mango) wrote:
>how does that work?
>
>susan

The original reference is:

Rackwitz HR; Zehetner G; Murialdo H; Delius H; Chai JH; Poustka A; Frischauf
A; Lehrach H 

Gene 40: 259-66 (1985) 

Abstract

A group of cosmid clones was isolated from the region of the mouse t complex and
    analysed by a rapid restriction mapping protocol based on linearization of
    circular cosmid DNA in vitro. A plasmid capable of producing high levels of
    phage lambda terminase was constructed and procedures for in vitro cleavage
    of cosmid DNAs were optimised. After linearization, the cosmids were
    partially digested with restriction enzymes, and either cos end was labelled
    by hybridization with radioactive oligos complementary to the cohesive end
    sequence, a step which we have described previously for clones in phage
    lambda (Rackwitz et al., 1984). High-resolution restriction maps derived by
    this method were used to identify and align the cosmids, to localise the
    position of repetitive sequences, and to interpret the results of electron
    microscopy heteroduplex experiments.


Jose Casal

MRC Laboratory of Molecular Biology        phone  +44 (0)1223 402282
Hills Road                                 fax    +44 (0)1223 412142
Cambridge CB2 2QH
UK

mailto:jec1 at mrc-lmb.cam.ac.uk



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