Gene Expression Protocols

Sean Blasdall sblasdal at bio.usyd.edu.au
Thu Jun 1 01:43:03 EST 1995


I am having problems expressing a pBluescript II clone, and the host is 
TOPP.


The insert is in frame.  I am using 1mM final concentration IPTG.
I am unable to detect the expressed protein on silver stain PAGE.


Any advice?





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