T cell stable electroporation conditions

batu erman erman at binah.cc.brandeis.edu
Thu Jun 1 12:51:32 EST 1995


Hi, if anyone is experienced in stably transfecting T cell lines (such as
9A10, AKR5, EL4, Jurkat, RLM11, AKR1G1) by electroporation, using neo
resistance, could you please direct me to a protocol for efficient
electroporation conditions? Also does anyone know when the lck promoter
turns on and off during development, and whether any of the abovementioned
cell lines express lck? 
Thank you. (I'll post the results of this search!)

Batu Erman



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