Cosmid purification

Mari L. Shinohara Mari.L.Shinohara at
Fri Jun 2 16:43:44 EST 1995

Hi netters,
I'm having a hard time purifying a cosmid clone.  
I understand that handling cosmids could be sometimes tough.  I always
streak clonies one day before purification, then Terrific Broth was
used for bacterial propagation.  I've got a small amount of cosmid with
Qiagen only once out of six trials:  Usually, I didn't get any. 
(Miniprep seems to work, but yield is terribly low.)

I would greatly appreciate if anyone can give suggestions.

Mari Shinohara
Dartmouth Medical School

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