Mari L. Shinohara
Mari.L.Shinohara at dartmouth.edu
Fri Jun 2 16:43:44 EST 1995
I'm having a hard time purifying a cosmid clone.
I understand that handling cosmids could be sometimes tough. I always
streak clonies one day before purification, then Terrific Broth was
used for bacterial propagation. I've got a small amount of cosmid with
Qiagen only once out of six trials: Usually, I didn't get any.
(Miniprep seems to work, but yield is terribly low.)
I would greatly appreciate if anyone can give suggestions.
Dartmouth Medical School
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