PCR errors:How many really?

Ted M tedm at darkwing.uoregon.edu
Fri Jun 2 17:00:11 EST 1995


In article <587243wnr at genesys.demon.co.uk>, Duncan at genesys.demon.co.uk wrote:

> In article: <1995May31.172756.85740 at ucl.ac.uk>  dmcbtch at ucl.ac.uk (Tom
Chappell) writes:
> 
> > sure they behave identically. (I'm doing some GFP things right now and
> > it's fairly easy to tell whether or not the GFP has been messed up in a
> > way that is critical to the experiment--it's not green...)
> 
> Could PCR and expression of GFP be used as a relative indicator of fidelity? 
> There isn't any easily available method to assess fidelity. You can use 
> Kunkel's phages.  Very accurate method but it doesn't look the simplest to
> set up. 
> Duncan 

Tom has done it!!! look it up !!                                Ted Michelini



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