Taq-EDTA

Duncan Clark Duncan at genesys.demon.co.uk
Fri Jun 2 03:40:14 EST 1995


In article: <3qkons$nsf at ceph.cephb.fr>  glibert at ceph.cephb.fr (Glibert Fabrice) writes:
> 
> from which concentration or amount does EDTA
> inhibits the Taq polymerase ?

EDTA itself doesn't inhibit the enzyme rather it chelates out the Magnesium
cofactor required for activity. As most Thermus pols require 1.5mM Mg for 
activity, EDTA above 0.1mM will cause problems. Addition of extra Mg will 
overcome the drop in activity.

Duncan 
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