[Q] human chr 20-specific library

NAKAMURA Yasukazu yanakamu at lab.nig.ac.jp
Sun Jun 4 22:41:48 EST 1995


Dear Bionetters, 

 Does anyone know human chr 20-specific genomic library constructor?

 I am a graduate student in Japan. I hope to use such a construction in
order to examine long-range GC content mosaic structure along the
chromosome.

 I know ATCC have two chr 20-specific constructions using Charon 21A
(57712, 57732).  Average insert size of them are 4.0kb and 3.1kb.  I want
to use library with larger (20kb or more) insert size.

 Any information will be appliciated. Thank you in advance.

YasKaz

NAKAMURA Yasukazu                yanakamu at lab.nig.ac.jp
Evolutionary Genetics Lab.       Grad. Univ. for Adv. Studies / NIG

P.S. If you have some interest in long-range GC content mosaic along the
human genome, see Fukagawa et al. "A boundary of long-range G+C% mosaic
domains in the human MHC locus: pseudoautosomal boundary-like sequence
exists near the boundary" Genomics 25, 184-191 (1995).



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