Nuclear Protein Prep. Chicken

Endoh, Daiji dendoh at vetmed.hokudai.ac.jp
Mon Jun 5 19:55:24 EST 1995


I'm about to isolate chicken embryo fibroblast nuclei, using the method of
Digman et al. (Methods in Enzymology vol.101) I am looking for the best
protocol to lyse these nuclei in order to obtain a NATIVE protein extract -
for use in gel-shift assays, SouthWestern blot analysis etc.
I would be most greatful for any guidance on this subject.
Thanks in advance,

Daiji Endoh
Hokkaido University




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