Nuclear Protein Prep.

Miss E.T. Tonkin etonkin at
Mon Jun 5 11:12:07 EST 1995

I'm about to isolate S.pombe yeast nuclei using the method of Aris and Blobel
(Methods in Enzymology vol.194), but I am looking for the best protocol to lyse
these nuclei in order to obtain a NATIVE protein extract - for use in gel-shift
assays, SouthWestern blot analysis etc.
I would be most greatful for any guidance on this subject.
Thanks in advance,

Emma Tonkin                        
University of Aberdeen

More information about the Methods mailing list