transfection variation w/luciferase

Brent Cochran bcochran_ypr at opal.tufts.edu
Mon Jun 5 11:01:10 EST 1995


We are having a lot of trouble with plate to plate variation with our 
transfections. The variation does not seem to be due to the transfection 
effeciency itself since the internal control SV-lacZ numbers vary less 
than 2 fold, whereas the luciferase numbers can vary 5-10 fold on 
duplicate plates. I suspect there is some problem with luciferase 
extraction and/or degradation.  Has anyone had this problem and know how 
to solve it?

-Brent





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