Mr. R. Cartwright
rcartwri at hgmp.mrc.ac.uk
Mon Jun 5 08:04:21 EST 1995
Does anyone know of a citation that describes why E.coli MC1061 cells containing the 60Kb P3 plasmid are so widely used in the generation of cDNA libraries. Is it just because the plasmid allows extra selection due to the amber mutations in the tetracycline and ampicilin genes or does the plasmid contain other sequences required for stable gene expression of the introduced cDNA molecules.
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