J.Parkhill at bham.ac.uk
Mon Jun 5 07:42:48 EST 1995
In article <crasmussen-0306951239500001 at mac05.biochem.ualberta.ca>,
crasmussen at anat.med.ualberta.ca (Colin Rasmussen) wrote:
> In article <cook_j-180595225230 at 22.214.171.124>, cook_j at bms.com (Joe Cook)
> > Does anyone know of a good cycle sequencing kit or method that incorporates
> > radioactively labelled nucleotides rather than end labelling of the primer?
> If I remember right, Promega's fmol system can be used either with
> labelled primers or with a labelled dNTP.
The Promega f-mol kit does work well with 35-S incorporation, and full
insructions are given with the kit.
No association with Promega, just a satisfied customer.
Julian Parkhill - J.Parkhill at bham.ac.uk
CRC Laboratories, Inst. of Cancer Studies, Medical School,
University of Birmingham, Edgbaston, Birmingham, B15 2TJ, U.K.
Disclaimer: If I thought anybody gave a damn about my opinions....
More information about the Methods