Molecular cloning -Sambrook et al/ Tris base vs TRIZMA

T.S. Pillay tpillay at
Sun Jun 4 19:24:01 EST 1995

In the electrophoresis section in Molecular Cloning by Sambrook et al 
(a.k.a. the "Maniatis")  it says not to use TRIZMA for SDS PAGE
electrophoresis buffers but Tris Base which should be pH'd to the
appropriate pH instead of using a mixture of Trisbase and Tris-HCl.  Does
anyone know the reason for this.

More information about the Methods mailing list