Does DraI cut ssDNA?
Juergen Sasse (Path)
js1 at mole.bio.cam.ac.uk
Mon Jun 5 03:48:47 EST 1995
On Wed, 31 May 1995, John Dixon wrote:
> Can anyone tell me if single stranded DNA can be cut by DraI?
> Or if any of the enzymes which cut 3' of their recognition site can cut
> (eg AlwI ...GGATCNNNN NNNNN...
> ...CCTAGNNNNN NNNN...)
> Cheers, Johnny D
> John Dixon Lab 44 (223) 334131
> Wellcome/CRC Institute Fax 44 (223) 334134
> Dept Genetics
> Cambridge University
Sorry, don't know about DraI. MnlI is an enzyme which cuts 3' of its
recognition sequence and ssDNA with 50 percent activity. This is litsted
in the new NEB catalog on page 212. They are the best people to ask for
further advice. Hope that helps.
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