Does DraI cut ssDNA?

Juergen Sasse (Path) js1 at mole.bio.cam.ac.uk
Mon Jun 5 03:48:47 EST 1995


On Wed, 31 May 1995, John Dixon wrote:

> Can anyone tell me if single stranded DNA can be cut by DraI?
>
> Or if any of the enzymes which cut 3' of their recognition site can cut
ssDNA?
> (eg AlwI ...GGATCNNNN    NNNNN...
>          ...CCTAGNNNNN    NNNN...)
>
> Cheers, Johnny D
>
> --
> John Dixon                     Lab 44 (223) 334131
> Wellcome/CRC Institute         Fax 44 (223) 334134
> Dept Genetics
> Cambridge University
>
>
Sorry, don't know about DraI. MnlI is an enzyme which cuts 3' of its
recognition sequence and ssDNA with 50 percent activity. This is litsted
in the new NEB catalog on page 212. They are the best people to ask for
further advice. Hope that helps.
Juergen






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