direct cloning of PCR product

mike roper you at somehost.HSC.Colorado.edu
Tue Jun 6 14:36:52 EST 1995


In article <3r1igt$6le at saphir.jouy.inra.fr>, marc at tours.inra.fr (Daniel.Marc) says:
>
>I have been told that some people make their own T/A-PCR cloning vectors by 
>simply incubating a blunt-ended, linearized vector, with dTTP and Taq 


See "Current Protocols in Molecular Biology"  There's a complete
description in it.

Cheers

:-)

Mike Roper
University of Colorado Health Sci. Ctr
Denver



More information about the Methods mailing list