DMSO for tissue culture use

Tim Dunn t.dunn at mailbox.uq.oz.au
Tue Jun 6 21:55:25 EST 1995


In article <D9G68v.Lvv at freenet.carleton.ca>, ar229 at FreeNet.Carleton.CA
(Allison Haggarty) wrote:

> We use DMSO as a solvent when adding retinoids to cultured cells.  Lately
> we have been noticing that the DMSO alone has been having a toxic effect
> on the cells.  Should we be using a special grade of DMSO (we have been
> using ACS grade from BDH)?  Any other suggestions to avoid cytotoxicity would
> be appreciated.  Thanks.
> 
DMSO oxidises over time.  I have noticed this cytotoxicity myself, and
started using DMSO packaged under N2 especially for cell culture purposes
(from SIGMA).  Problem solved.

-- 
Tim Dunn
Centre for Molecular and Cellular Biology
University of Queensland
Australia 4075
e-mail t.dunn at mailbox.uq.oz.au
phone  (07) 365 4565
fax    (07) 365 4388 



More information about the Methods mailing list