Help, decreasing temp. during long sequencing runs!
ovanham at julian.uwo.ca
Tue Jun 6 15:12:00 EST 1995
I've been finding that when I run my sequencing gel for relatively long
runs, the temperature (and current) steadily drops. I've heard that this
is due to a pH change in the buffer (TBE). Is this true? If so, how do I
remedy the problem. The situation is complicated by the fact that the
buffer is hot (P32) from the samples.
Thanks in advance for any suggestions.
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