Help, decreasing temp. during long sequencing runs!

o. van-ham ovanham at julian.uwo.ca
Tue Jun 6 15:12:00 EST 1995


I've been finding that when I run my sequencing gel for relatively long 
runs, the temperature (and current) steadily drops.  I've heard that this 
is due to a pH change in the buffer (TBE).  Is this true?  If so, how do I 
remedy the problem.  The situation is complicated by the fact that the 
buffer is hot (P32) from the samples.

Thanks in advance for any suggestions.

Oded



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