Does DraI cut ssDNA?
jpcd0 at mole.bio.cam.ac.uk
Tue Jun 6 08:22:02 EST 1995
In article <3qugdf$ge1 at mserv1.dl.ac.uk>, "Juergen Sasse (Path)"
<js1 at mole.bio.cam.ac.uk> wrote:
> On Wed, 31 May 1995, John Dixon wrote:
> > Can anyone tell me if single stranded DNA can be cut by DraI?
I just tried this myself and I didn't work appreciably...
> Sorry, don't know about DraI. MnlI is an enzyme which cuts 3' of its
> recognition sequence and ssDNA with 50 percent activity. This is litsted
> in the new NEB catalog on page 212.
Mnl1 recognizes 5' ...CCTC NNNNNNN NNNNN... 3'
3' ...GGAG NNNNNN NNNNNN... 5'
on double stranded DNA. So on ssDNA it presumably cuts:
5' ...CCTC NNNNNNN NNN...3'
but will it also cut at
5' ...NNNNNN NNNNNN GAGG... 3' (ie the recognition site on the lower
strand o of the double stranded sequence
John Dixon Lab 44 (223) 334131
Wellcome/CRC Institute Fax 44 (223) 334134
United Kingdom e-m: jpcd0 at mole.bio.cam.ac.uk
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