Molecular cloning -Sambrook et al/ Tris base vs TRIZMA

Denni Schnapp ds4 at st-andrews.ac.uk
Wed Jun 7 05:22:03 EST 1995


We use Sigma TRIZMA for all SDS PAGE (Laemmli and Schaegger & von Jagow
methods) but it is indeed the same as Tris base. Buffers are prepared by dissolving an amount of Tris in deionized water (the higher the pH the more Tris),
then titrating with conc. HCL. Take note that a special electrode is required
for titrating Tris buffers because the Tris can penetrate and muck up ordi-
nary pH electrodes.

Denni (ds4 at st-and.ac.uk)



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