cDNA-synthesis Kits

Hiranya Roychowdhury hroychow at NMSU.EDU
Wed Jun 7 12:48:52 EST 1995

On 7 Jun 1995, Artur da Camara Machado wrote:

>               What is the best Kit available for 
>               synthesising  cDNA for cloning?
>               (fast, full-length etc)
>                                                         Thanks Georg Seifert
There is no kit that is BEST for cDNA cloning. As far as I could tell 
from the experiences of a number of my colleagues, the success of the 
kits depend on the system on which they are used. For example, one of my 
friends could never get a perticular kit to work on the poly(A+) of an 
ascomycete, while another used the same kit and constructed a good 
library from Brassica napus flowers. A better result was obtained by this 
same friend when he used a kit from another supplier (I think it was 
Amersham; usual disclaimer). I have never used a kit just for the fear of 
not being able to get the proper library.

			  Hiranya S. Roychowdhury
   			  Plant Genetic Engineering Lab.
			  Box 3GL, NM State Univ.
			  Las Cruces, NM 88003
			  Phone: (505) 646-5785
			  hroychow at

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