UNDER HUGE PRESSURE ?

[Claude Aflalo]

On Wed, 7 Jun 1995 Martin at GDB160.uk.sb.com wrote:

> 
> Does anyone know where on this planet I can purchase a Hughes Pressure Cell ?
> It's a stainless steel block with two cylinders in it joined by a narrow 
> aperture. The idea is that one  cools it to -20deg' C say, puts a suspension of 
> bugs or whatever into one cylinder and by application of pressure to a piston 
> pushes the solid pellet through the aperture. This shears the material in the 
> pellet. I want it because I wish to crack open  pathogenic bacteria in small 
> volumes and for this would be more suitable than a French press for example. 
> No, my surname isn't Hughes!
> Thanks for any forthcoming advice,
> Martin. 
> 

Hello Martin, 
You'll probably can find this kind of device under different names: It 
used to be called "French press" or also "Yeda press". These do not use a 
piston to apply pressure but compressed Nitrogene or Helium. They are 
used for the preparation of sub-mitochondrial, sub-chloroplast, 
bacterial chromatophores, or a variety of sub cellular particles. Try the 
Thomas catalog. 

Claude Aflalo ###########################################################
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