calculating Tm with formamide

John Dixon jpcd0 at
Thu Jun 8 09:02:13 EST 1995

Hi, can anyone let me know where I can find an equation for calculating
the Tm of primers when the buffer is supplemented with varying percentages
of formamide?
No luck in Maniatis or practical approach.

Cheers, Johnny D

John Dixon                     Lab 44 (223) 334131
Wellcome/CRC Institute         Fax 44 (223) 334134
Dept Genetics
Cambridge University    
United Kingdom       e-m: jpcd0 at

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