Transcriptionally active Nuclear Extracts

bd
Thu Jun 8 19:37:39 EST 1995


Does anybody know of a good protocol for making
nuclear extracts that can be used for in vitro transcription
assays from Monolayer cells, not suspension cells like HeLa??
I am interested in how many cells  to use (eg. cells/100 mm dish)
Also, does scraping the cells off the dishes kill a lot of cells?

I would appreciate any input !!!


Thanks a lot,
-damania



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