Transcriptionally active Nuclear Extracts

Thu Jun 8 19:37:39 EST 1995

Does anybody know of a good protocol for making
nuclear extracts that can be used for in vitro transcription
assays from Monolayer cells, not suspension cells like HeLa??
I am interested in how many cells  to use (eg. cells/100 mm dish)
Also, does scraping the cells off the dishes kill a lot of cells?

I would appreciate any input !!!

Thanks a lot,

More information about the Methods mailing list