calculating Tm with formamide

sjgouldrulesmiggy baldoni.1 at postbox.acs.ohio-state.edu
Thu Jun 8 11:46:27 EST 1995


In article <jpcd0-0806951402130001 at macr1-6.welc.cam.ac.uk>,
jpcd0 at mole.bio.cam.ac.uk (John Dixon) wrote:

> Hi, can anyone let me know where I can find an equation for calculating
> the Tm of primers when the buffer is supplemented with varying percentages
> of formamide?
> No luck in Maniatis or practical approach.
> 
> Cheers, Johnny D
> 
> -- 
> John Dixon                     Lab 44 (223) 334131
> Wellcome/CRC Institute         Fax 44 (223) 334134
> Dept Genetics
> Cambridge University    
> United Kingdom       e-m: jpcd0 at mole.bio.cam.ac.uk


John,
   Hopes this helps! It is known as the %GC method:


Tm[celcius]=
81.5+16.6*log[Na]+0.41*(%GC)-675/length-0.65*(%formamide)-(%mismatch)

regards,
miggy

-- 
"The vice of our country is that we are always outraged at the wrong things"......Norman Mailer



More information about the Methods mailing list