calculating Tm with formamide

sjgouldrulesmiggy baldoni.1 at
Thu Jun 8 11:46:27 EST 1995

In article <jpcd0-0806951402130001 at>,
jpcd0 at (John Dixon) wrote:

> Hi, can anyone let me know where I can find an equation for calculating
> the Tm of primers when the buffer is supplemented with varying percentages
> of formamide?
> No luck in Maniatis or practical approach.
> Cheers, Johnny D
> -- 
> John Dixon                     Lab 44 (223) 334131
> Wellcome/CRC Institute         Fax 44 (223) 334134
> Dept Genetics
> Cambridge University    
> United Kingdom       e-m: jpcd0 at

   Hopes this helps! It is known as the %GC method:



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