Total RNA quantitation

hfakhour at fs1.ho.man.ac.uk
Thu Jun 8 11:55:32 EST 1995


Hello

I am extracting total RNA from a small number of cells (< million)  using 
Ultraspec RNA reagent. The yield is too low to be measured accurately by 
spectrophotometry.Does anyone have any suggestions for accurate 
measurment of small amount of total RNA concentration (< 5ug)
to be used for quantitative RT-PCR? Help is very much  appreciated.




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