Being immortalized ?
wmoens at ben.vub.ac.be
Fri Jun 9 11:39:42 EST 1995
Primary cultures of mammalian cells usually die after a certain number of passag
es in vitro. In rodent primaries, some cells may survive to the crisis and behav
e as "immortal". This extremely rarely happens with human primaries.
Therefore "immortalisation" vectors are used to get immortalisation of human
In fact I have difficulties to catch exactly on which physiological/molecular fa
cts one can declare a successful "immortalisation" of such cultures.
Are they mutants of apoptosis, then which are the criteria to check?
Are they surviving cells with or without normal cell cycling rates?
More information about the Methods