RNA ext. from vessel endothelial cells
Garry P. Larson
glarson at ccmail.llu.edu
Fri Jun 9 20:38:36 EST 1995
Can any1 give me a good protocol for preparing RNA from arterial
endothelial cells directly from mammals? The endothelial cells comprise
perhaps only 10% of the total cell population compared to connective tissue
cells. Can one just use a Qiagen RNA extraction kit (or similar company
product) after grinding the heck out of the tissue? Or is there a better
way? Do you have any protocols? I'd sure like some good recommendations.
Plz email me the ans. as I don't get to visit these parts often. OK?
If "The truth is out there", I must be looking in the wrong place!
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