Help! Quantification of total RNA, equal loading
Aron B. Jaffe
jaffe at a1.mscf.upenn.edu
Fri Jun 9 11:40:33 EST 1995
crasmussen at anat.med.ualberta.ca (Colin Rasmussen) wrote:
> In article <3r7b1n$rpr at netnews.upenn.edu>, ljohnson at pobox.upenn.edu (Lois
> Johnson) wrote:
> > Just a question... Can you strip RNA off a blot when you strip off the
> > first probe?
> > Lois Johnson
> > ljohnson at pobox.upenn.edu
> You tend to lose a little, but we have re-probed blots more than 5 times
> and not had any problems with significant loss.
The amount of RNA that strips off the blot depends on the material
that you blot on... nitrocellulose seems to be the worst when trying
to rehybridize a blot after stripping the probe. Our lab uses
Nylon and it works well for several hybridiziations.
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