Change in Mol. wt.
SaiSu at MCB.LiU.SE
Sat Jun 10 07:51:21 EST 1995
>To: methods-and-reagents at net.bio.net
>From: A.C.Hilton at bham.ac.uk (Wiggy)
>Subject: Change in Mol. wt.
>Date: 9 Jun 1995 15:23:12 GMT
>I recently recovered a fragment from an agarose gel by a freeze squeeze
>method and re-ran a small amount on an acrylamide gel to check what I had
>got back. The band in the agarose gel according to a 1kb ladder marker was
>about 750-800bp. The recovered fragment in the 5% acrylamide gel
>according to the same markers appeared to be just greater then 1kb!
>Any suggestions as to what is going on?
>Anyone seen this in their gels?
Yep! it has happened to me also, I was using Pharmacia's band prep and my
fragments size increased, thought due to be an error since these bands have
cohesive ends, but the same never happened when using Quiaex II protocol, could
also be due to the short UV exposure instead of long UV. Pls let me also know if
you happened to find a solution.
Growing OLD may be manditory
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Staying Young Forever..
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