Edman degradation of bacterial proteins

James Bassuk bassuk at u.washington.edu
Sat Jun 10 21:17:20 EST 1995


The N-f-Met will be cleaved by a bacterial peptidase before you have a
chance to isolate it.  It should not be a consideration.

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   Jim Bassuk, Ph.D.
   Dept. Biological Structure, Univ. of Washington
   NetworkAddress:  Bassuk at U.Washington.Edu  

   'Did I say what I meant or did I mean what I said?'

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On 
Wed, 7 Jun 1995, Jan Eggermont wrote:

> Hello,
> 
> A small question for protein sequencers who have experience with Edman
> degradation of bacterial proteins: is the N-formyl-methionine, i.e. the
> modified start methione of bacterial proteins, derivatised in one single
> cycle or in two, i.e. first the formyl and then the methionine? 
> Thanks,
> 
> Jan
> 
> -- 
> Jan Eggermont                              Jan.Eggermont at med.kuleuven.ac.be
> Laboratorium voor Fysiologie - KU Leuven
> Campus Gasthuisberg                        tel 32-16-34 59 38
> 3000 Leuven     Belgium                    fax 32-16-34 59 91
> 
> 



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