Uh-oh, open freezer door...

Martin Kennedy mkennedy at chmeds.ac.nz
Mon Jun 12 06:03:04 EST 1995


In article <3rad56$p5r at amy26.Stanford.EDU>, ladasky at leland.Stanford.EDU (John Ladasky) writes:
> Greetings, folks,
> 
> 	The other day I came in to the lab to disocver that our -20 C
.. stuff deleted...
> hours.  My question is: what are our chances?  Can we use these enzymes
> or will we find that they have been largely inactivated?  I would be 
> grateful if people whould share their experience.
> 
> -- 
> Unique ID : Ladasky, John Joseph Jr.

I would only be concerned about Sequenase or Klenow; most other 
enzymes ought to be fine, and even these 2 may be ok - but bear 
it in mind if you get a blank sequencing gel next run!

Cheers,

Martin

NNNN   NN  Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz)  ZZZZZZZ  
NN NN  NN       Cytogenetic and Molecular Oncology Unit          ZZZ
NN  NN NN           Christchurch School of Medicine            ZZZ
NN   NNNN              Christchurch, New Zealand              ZZZZZZZ
		Phone (64-3)364-0880   Fax (64-3)364-0750



More information about the Methods mailing list