Phenol/Chlo/EtOH

Shin-ichiro HIRAGA shiraga at gen-info.osaka-u.ac.jp
Sun Jun 11 04:26:53 EST 1995


One of my collaborator had same problem before. In his case, the volume of TE or H20 used for 
suspending the dried DNA attached to the side wal of the microfuge tube. So most part of the DNA 
recovered was left on the wall as dried.

Although 20ul seems to enough for suspending, if the volume of EtOH ppt is much larger, the problem 
might arise.

Good Luck.

Shin-ichiro Hiraga
Lab. of Genetics
Dept. of Biology
Fac. of Sci.
Osaka University
Japan



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