cDNA from small quantity of RNA?

zinetti at zinetti at
Mon Jun 12 18:17:25 EST 1995

>   m.hubank at (Mike Hubank) writes:
>  I need to make double stranded cDNA from fairly low amounts of starting
>  RNA. I have been trying synthesis direct from total RNA (>5ug), but with
>  little apparant success. Does anybody have a relatively reliable protocol
>  for this sort of thing?
>  Thanks
>  Mike Hubank
>  m.hubank at
Dynal co.  The maker of Dynabeads, I have tried this protocol 2 times, without success, but it may be my own fault.
Good Luck

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