multiple bands on 2nd rd PCR

Edward Wang ez022056 at dale.ucdavis.edu
Mon Jun 12 16:00:52 EST 1995


I was wondering anybody had this problem...I am PCRing bands that were 
extracted from acrylamide gel.  Some of them show up with the first round 
of PCR.  Others require a second round of PCR.  However, after the second 
round, some of my products had multiple bands (2-4).  Does anybody have 
any explaination for this other then contamination?
Thanks in advance...

--
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Edward H. Wang                 *
Staff Resch. Assc.             *
Dept. Internal Med. -Nephrology*
UC Davis                       *
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