help: in vitro transcription

Lars Thelander lars.thelander at medkem.umu.se
Tue Jun 13 06:58:01 EST 1995


Does anyone know of a good reference for RNase protection assay to measure the amount of RNA in 
from an in vitro transcription assay? Can this readily be done by making a riboprobe (off a T7 
promoter) and labeling with 35SUTP (or another rNTP) or do I need to use 32P? Also, I am 
planning to label RNA with 32P off a linearized plasmid with my promoter of interest but am 
open to alterantives. I am just starting in vitro transcription so any help would be most 
appreciated.

Thank you.

Eric Hanson




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