[q]Cloning Mycoplasma DNA ?

u2528090 at ucsvc.ucs.unimelb.edu.au u2528090 at ucsvc.ucs.unimelb.edu.au
Wed Jun 14 20:53:52 EST 1995


Hi,

I'm working on cloning Mycoplasma genes into E. coli, and
am having little success.

I'm having problems making a Hind III library of M. hyopneumoniae
dna ligated into pBluescript II and electroporated into XL1 Blues.

Does anyone know if XL1 Blues have a problem with Mycoplasma DNA
( high AT content about 70 % )?

thanks
Stephen U2528090 at hermes.ucs.unimelb.edu.au




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