TAE or TBE

[Andy Kravetz]

Edwin ten Dam (ebtd1 at cam.ac.uk) wrote:

: 2. If isolating a restiction fragment from a gel for cloning purposes, use
: TAE (TBE will give lower cloning yields, depending on your clean-up
: procedure)

Why is that? I use TBE for all my gels but LMP agarose and find that TBE 
is more consistent and you can run it much hotter and faster. But I do a 
lot of cloning so I am anxious to hear your reasoning.

thanks

andy



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Andy Kravetz
St. Louis, Missouri
akravetz at mo.net