DGGE STAINING PROBLEM
SPRASHAR at EM.AGR.CA
Wed Jun 14 07:28:28 EST 1995
Does anybody know why acrylamide gels won't stain with eithidium
bromide but the same gel will stain nicely with silver stain.
Conditions of gel running are overnight at 60 C. Buffer being used
is TAE pH 7.4. (Buffer recirculates during the run by means of
Imp: Using the same conditions these DGGE gels used to stain
We have tried changing :Fresh stain 0.5ug/ml.
:Prepared all reagents from brand
new chem. bottles.
:Double checked the pH of Buffer.
NOW I AM LOOKING FOR ANY SUGGESTIONS YOU HAVE.
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